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multiplex rt-pcr detection and distribution of four apple viruses in china.apple trees are natural hosts of four economically important virus species in china. we used a simple, sensitive multiplex rt-pcr protocol with an internal control to simultaneously detect and differentiate four apple viruses: apple chlorotic leaf spot virus (aclsv), apple mosaic virus (apmv), apple stem pitting virus (aspv), and apple stem grooving virus (asgv). this multiplex rt-pcr could be used as an alternative to other routinely used detection methods. we used this protocol to evaluate the ...201324294957
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