| multiplex rt-pcr detection and distribution of four apple viruses in china. | apple trees are natural hosts of four economically important virus species in china. we used a simple, sensitive multiplex rt-pcr protocol with an internal control to simultaneously detect and differentiate four apple viruses: apple chlorotic leaf spot virus (aclsv), apple mosaic virus (apmv), apple stem pitting virus (aspv), and apple stem grooving virus (asgv). this multiplex rt-pcr could be used as an alternative to other routinely used detection methods. we used this protocol to evaluate the ... | 2013 | 24294957 |
| complete nucleotide sequences of the genomes of two isolates of apple chlorotic leaf spot virus from peach (prunus persica) in china. | the complete nucleotide sequences of two isolates of apple chlorotic leaf spot virus (z1 and z3) collected from peach in henan province, china, were determined. the genomes of both z1 and z3 were found to contain three open reading frames (orfs). sequence analysis showed that genomic sequences of z1 and z3 isolates shared 67.4%-82.9% and 67.2%-82.6% identity, respectively, with the other eight isolates of aclsv that have been reported previously. based on the putative amino acid sequences of the ... | 2012 | 22278708 |