| characterization and genus identification of rhizobial symbionts from caragana arborescens in western canada. | naturally occurring nitrogen-fixing symbionts from root nodules of caragana (caragana arborescens) growing in central saskatchewan were isolated following surface sterilization of caragana root nodules and squashing and spreading of the contents on yeast extract - mannitol medium. the symbiotic nature of the strains was confirmed following inoculation onto surface-sterilized c. arborescens seed in a gnotobiotic leonard jar system. the rhizobium isolates from c. arborescens root nodules were inte ... | 2013 | 23750954 |