| detection of mycobacterium avium subsp. paratuberculosis in bovine milk from the state of pernambuco, brazil. | the aim of this study was to detect the is900 region of mycobacterium avium subsp. paratuberculosis (map) in bovine milk samples using real-time polymerase chain reaction (qpcr) and conventional pcr, and to study the agreement between these tests. a total of 121 bovine milk samples were collected from herds considered positive for map, from the state of pernambuco, brazil. map dna was detected in 20 samples (16.5%) using conventional pcr and in 34 samples (28.1%) using qpcr. map dna was detected ... | 2017 | 27865632 |