| evaluation and molecular characterization of human adenovirus in drinking water supplies: viral integrity and viability assays. | human adenoviruses (hadvs) are the second-leading cause of childhood gastroenteritis worldwide. this virus is commonly found in environmental waters and is very resistant to water disinfection and environmental stressors, especially uv light inactivation. molecular techniques, such as pcr-based methods (polymerase chain reaction), are commonly used to detect and identify viral contamination in water, although pcr alone does not allow the discrimination between infectious and non-infectious viral ... | 2013 | 23714224 |
| surveillance of human and swine adenovirus, human norovirus and swine circovirus in water samples in santa catarina, brazil. | animal and human wastewater can potentially contaminate water sources and the treatment of drinking water may not effectively remove all contaminants, especially viruses. the purpose of the present study was to evaluate the viral contamination of water used for human and animal consumption in the city of concórdia, located in southern brazil. porcine circovirus type 2 (pcv2), porcine adenovirus (padv), human adenovirus (hadv) and human norovirus (nov) were searched for using quantitative polymer ... | 2012 | 22960488 |
| surveillance of human viral contamination and physicochemical profiles in a surface water lagoon. | the present study evaluated the contamination of a surface water lagoon (peri lagoon) in florianópolis, santa catarina, brazil, by human adenovirus (hadv), polyomavirus jc (jcpyv), hepatitis a virus (hav) and rotavirus species a (rva). efforts were driven to determine the correlation between viral presence and the physicochemical parameters of the lagoon and measure the distribution of these viruses throughout the year (june 2010 to may 2011). a total of 48 samples were collected, concentrated a ... | 2012 | 23109586 |
| enteric viruses in surface water and sediment samples from the catchment area of peri lagoon, santa catarina state, brazil. | this paper aims to quantify human adenovirus (hadv), rotavirus species a (rva), and hepatitis a virus (hav) in surface water and sediments and to determine the viability of hadv in these samples. water and sediment samples were collected, and hadv, rva, and hav were quantified by real-time polymerase chain reaction (pcr); hadv was also evaluated for infectivity by a plaque assay (pa). for the water samples, hadv was detected in 70.8% of the summer collections, with 82.4% containing infectious ha ... | 2016 | 26837838 |
| spatial distribution of enteric viruses and somatic coliphages in a lagoon used as drinking water source and recreation in southern brazil. | this study aimed to evaluate the contamination level of the peri lagoon, the main freshwater reservoir of santa catarina island, southern brazil, for human adenovirus (hadv), hepatitis a virus (hav), rotavirus species a (rva), and somatic coliphages (somcph). viruses were also investigated in sediments and their sensitivity against natural sunlight was analysed by studying their spatial distribution in different depths of the water column. a total of 84 water samples and 48 sediment samples were ... | 2016 | 27449740 |
| evaluation of tropical water sources and mollusks in southern brazil using microbiological, biochemical, and chemical parameters. | florianópolis, a city located in the santa catarina state in southern brazil, is the national leading producer of bivalve mollusks. the quality of bivalve mollusks is closely related to the sanitary conditions of surrounding waters where they are cultivated. presently, cultivation areas receive large amounts of effluents derived mainly from treated and non-treated domestic, rural, and urban sewage. this contributes to the contamination of mollusks with trace metals, pesticides, other organic com ... | 2012 | 22036209 |
| human and animal enteric virus in groundwater from deep wells, and recreational and network water. | this study was designed to assess the presence of human adenovirus (hadv), rotavirus-a (rva), hepatitis a virus (hav), and porcine circovirus-2 (pcv2) in groundwater from deep wells, and recreational and network waters. the water samples were collected and concentrated and the virus genomes were assessed and quantified by quantitative pcr (qpcr). infectious hadv was evaluated in groundwater and network water samples by integrated cell culture using transcribed messenger rna (mrna) (icc-rt-qpcr). ... | 2015 | 26300358 |