| introduction of the dengue virus type 4 in the state of mato grosso do sul, brazil. | we report on the first isolation of dengue virus serotype 4 (denv-4) in the state of mato grosso do sul, brazil, in february, 2012. the cases were isolated in the city of campo grande, the state capital, and presented the classic signs and symptoms of dengue fever. denv-4 was primarily identified through viral isolation in c6/36 clone lineage of aedes albopictus cells; followed by indirect immunofluorescence, using type-specific monoclonal antibodies. the results were subsequently confirmed by n ... | 2014 | 25210917 |