| use of a real time pcr for detecting subspecies of babesia canis. | this paper reports the development and use of a real time pcr for detection of babesia canis canis, b. canis rossi, and b. canis vogeli in endemic areas of brazil. the sequences of the internal transcribed spacer (its) of several organisms were aligned and five primers and four probes were designed for amplification of a fragment (around 125 bp) which differentiates subspecies of b. canis. blood samples collected from dogs living in farms in three distinct rural regions within the state of minas ... | 2012 | 22516643 |