| kinetics of poliovirus shedding following oral vaccination as measured by quantitative reverse transcription-pcr versus culture. | amid polio eradication efforts, detection of oral polio vaccine (opv) virus in stool samples can provide information about rates of mucosal immunity and allow estimation of the poliovirus reservoir. we developed a multiplex one-step quantitative reverse transcription-pcr (qrt-pcr) assay for detection of opv sabin strains 1, 2, and 3 directly in stool samples with an external control to normalize samples for viral quantity and compared its performance with that of viral culture. we applied the as ... | 2015 | 25378579 |