effect of temperature on symptom expression and sequence polymorphism of grapevine yellow speckle viroid 1 in grapevine. | symptom expression of yellow speckle disease was studied in a row of 32 individual vitis vinifera cv. chardonnay vines in the warmer summer of 2009/2010 as compared with the cooler summer of 2011/2012 in south australia. rt-pcr analysis showed that all these vines were positive for hop stunt viroid, grapevine yellow speckle viroid 1 (gysvd-1) and grapevine rupestris stem pitting-associated virus. four vines named vines 1, 8, 11 and 15 were selected for further analyses. vines 1 and 8 had never s ... | 2014 | 24905287 |