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isolation and identification of legionella and their host amoebae from weak alkaline carbonate spring water using a culture method combined with pcr.legionella were detected with the direct dna extraction method, legionella culture method, and free-living amoebae (fla) culture method from weak alkaline carbonate spring water in taiwan. moreover, we also investigated the existence of acanthamoeba, hartmannella, and naegleria, ubiquitous fla in aquatic environments, to identify the correlations between existing legionella. this study reports detecting legionella in 15 of the 51 weak alkaline carbonate spring water samples (29.4%). this work al ...201121537990
surveillance and evaluation of the infection risk of free-living amoebae and legionella in different aquatic environments.free-living amoebae (fla) are ubiquitous in various aquatic environments. several amoebae species are pathogenic and host other pathogens such as legionella, but the presence of fla and its parasites as well as the related infection risk are not well known. in this study, the presence of pathogenic fla and legionella in various water bodies was investigated. water samples were collected from a river, intake areas of drinking water treatment plants, and recreational hot spring complexes in centra ...201425192927
differential legionella spp. survival between intracellular and extracellular forms in thermal spring environments.legionella are commonly found in natural and man-made aquatic environments and are able to inhabit various species of protozoa. the relationship between the occurrence of legionella spp. within protozoa and human legionellosis has been demonstrated; however, the proportions of intracellular and extracellular legionella spp. in the aquatic environment were rarely reported. in this study, we developed a new method to differentiate intracellular and extracellular legionella spp. in the aquatic envi ...201323054762
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