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dietary korean mistletoe enhances cellular non-specific immune responses and survival of japanese eel (anguilla japonica).the present study was performed to investigate the immunostimulatory effects of korean mistletoe extract (km-110; viscum album coloratum) on the non-specific immune response and protection against aeromonas hydrophila infection in japanese eel (anguilla japonica). eels were fed under 4 regimes, 0%, 0.1%, 0.5% and 1.0% km-110 mixed diet. on day 14 after feeding, 15 fish from each group were injected i.p. with live a. hydrophila (3 x 10(6)cfu) and the remaining unchallenged fish from each group we ...200818023593
the in vitro effect of bovine lactoferrin on the activity of organ leukocytes in rainbow trout (oncorhynchus mykiss), european eel (anguilla anguilla) and wels catfish (silurus glanis).lactoferrin (lf) is a glycoprotein found in milk, neutrophil granules, secretions and selected organs of mammals. lactoferrin exhibits antibacterial, antiviral, fungicidal, immunoregulatory and other functions. although fish are devoid of this protein and its cell receptors, lf effect on the immune mechanisms of fish has been demonstrated. the objective of this study was to investigate the effect of bovine lactoferrin, applied in vitro, on the activity of head kidney and spleen leukocytes in thr ...201021077435
enhancement of protective immunity in european eel (anguilla anguilla) against aeromonas hydrophila and aeromonas sobria by a recombinant aeromonas outer membrane protein.to develop a vaccine, which can simultaneously prevent the diseases caused by various pathogenic bacteria in fish, we try to find a conserved outer membrane protein (omp) antigen from different bacterial pathogens. in this study, an omp fragment of 747 bp (named as omp-g), which was highly conserved in seven aeromonas omp sequences from the ncbi database, was amplified by pcr from one aeromonas sobria strain (b10) and two aeromonas hydrophila strains (b27 and b33) with the designed specific prim ...201021148192
bacterial pathogens and flora isolated from farm-cultured eels (anguilla japonica) and their environmental waters in korean eel farms.the purpose of this study was to investigate bacterial pathogens and flora in both sick and clinically healthy eels, anguilla japonica, and the environmental rearing waters of korean eel farms. between 2003 and 2010, a total of 621 sick eels were submitted for diagnosis, while 216 healthy eels and 87 environmental water samples were collected during a survey of 26 eel farms in korea. seven different bacterial species were obtained from 183 isolates, which were recovered from the internal organs ...201223357049
identification and characterization of a novel conserved 46 kd maltoporin of aeromonas hydrophila as a versatile vaccine candidate in european eel (anguilla anguilla).european eel (anguilla anguilla) is a crucial economic fish that has been plagued by aeromonas hydrophila infections for many years. vaccines that are cross-protective against multiple serotypes could provide an effective control against a. hydrophila-mediated diseases. the outer membrane proteins (omps) are highly immunogenic and capable of eliciting protective immune responses. this study reports the identification of a novel 46 kd maltoporin that is a conserved protective antigen for differen ...201728279793
ribose operon repressor (rbsr) contributes to the adhesion of aeromonas hydrophila to anguilla japonica mucus.the characterization of adhesion between pathogenic bacteria and the host is critical. pathogenic aeromonas hydrophila was shown to adhere in vitro to the mucus of anguilla japonica. to further investigate the adhesion mechanisms of a. hydrophila, a mini-tn10 transposon mutagenesis system was used to generate an insertion mutant library by cell conjugation. seven mutants that were impaired in adhesion to mucus were selected out of 332 individual colonies, and mutant m196 was the most severely im ...201728127946
flagellar motility is necessary for aeromonas hydrophila adhesion.adhesion to host surface or cells is the initial step in bacterial pathogenesis, and the adhesion mechanisms of the fish pathogenic bacteria aeromonas hydrophila were investigated in this study. first, a mutagenesis library of a. hydrophila that contained 332 random insertion mutants was constructed via mini-tn10 km mutagenesis. four mutants displayed the most attenuated adhesion. sequence analysis revealed that the mini-tn10 insertion sites in the four mutant strains were flgc(genbank accession ...201627432325
a novel recombinant bivalent outer membrane protein of vibrio vulnificus and aeromonas hydrophila as a vaccine antigen of american eel (anguilla rostrata).the immogenicity of a novel vaccine antigen was evaluated after immunized american eels (anguilla rostrata) with a recombinant bivalent expressed outer membrane protein (omp) of vibrio vulnificus and aeromonas hydrophila. three groups of eels were intraperitoneal (i.p) injected with phosphate-buffered saline (pbs group), formaline-killed-whole-cell (fkc) of a. hydrophila and v. vulnificus (fkc group) or the bivalent omp (omp group). on 14, 21, 28 and 42 days post-vaccination respectively, prolif ...201525655329
immune effects of a bivalent expressed outer membrane protein to american eels (anguilla rostrota).the specific and non-specific immune parameters and protection of american eels (anguilla rostrata) were evaluated after immunized eels with a bivalent expressed out membrane protein (omp) of porin ⅱ of aeromonas hydrophila and omps2 of edwardsiella tarda. one hundred eighty eels were distributed into 3 equal groups and intraperitoneal (i.p) injection with phosphate-buffered saline (pbs group), formalin-killed-whole-cell (fkc) of a. hydrophila and e. tarda (fkc group) or the bivalent omp (omp gr ...201323643876
flagellar motility contributes to the invasion and survival of aeromonas hydrophila in anguilla japonica macrophages.the interaction between pathogenic bacteria and the host phagocytes is complicated. it is generally believed that only obligate intracellular pathogens can invade and survive in host phagocytes. in this study, we revealed that the pathogenic aeromonas hydrophila b11 can also invade and survive in the macrophages of its host anguilla japonica in vitro. to further investigate the mechanisms of a. hydrophila invasion and survival in host macrophages, a mini-tn10 transposon mutagenesis system was us ...201424859591
mind plays an important role in aeromonas hydrophila adherence to anguilla japonica mucus.for a better understanding of the genitic regulation of the adhesion of aeromonas hydrophila, a mini-tn10 transposon mutagenesis system was introduced to generate an insertion mutant library by cell conjugation between the donor escherichia coli sm10 (plof/km) and the recipient a. hydrophila strain w. out of 332 individual colonies, 7 mutants with significantly attenuated adhesion ability were selected. the dna sequence flanking the mini-tn10 transposon inserted in the mutant strain m45 was ampl ...201525881868
hemerythrin is required for aeromonas hydraphlia to survive in the macrophages of anguilla japonica.survival in host phagocytes is an effective strategy for pathogenic microbes to spread. to understand the mechanisms of aeromonas hydrophila survival within host macrophages, a library of mini-tn10 transposon insertion mutants was constructed. the m85 mutant, whose survival in host macrophages was only 23.1% of that of the wild-type (wt) strain, was utilized for further study. molecular analysis showed that a 756-bp open reading frame (orf) (genbank accession no. cp007576) in the m85 mutant was ...201627173333
identification of a retinoic acid-inducible gene i from japanese eel (anguilla japonica) and expression analysis in vivo and in vitro.rig-i (retinoic acid inducible gene-i) is one of the key cytosolic pattern recognition receptors (prrs) for the recognition of cytosolic viral nucleic acids and the production of type i interferons (ifns). the full-length cdna sequence of rig-i (ajrig-i) in japanese eel (anguilla japonica) was identified and characterized in this article. the full-length cdna of ajrig-i was 3468 bp, including a 5'-untranslated region (utr) of 52 bp, a 3'-utr of 617 bp and an open reading frame (orf) of 2799 bp e ...201627238428
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