| metallothionein from wild populations of the african catfish clarias gariepinus: from sequence, protein expression and metal binding properties to transcriptional biomarker of metal pollution. | anthropogenic pollution with heavy metals is an on-going concern throughout the world, and methods to monitor release and impact of heavy metals are of high importance. with a view to probe its suitability as molecular biomarker of metal pollution, this study has determined a coding sequence for metallothionein of the african sharptooth catfish clarias gariepinus. the gene product was recombinantly expressed in escherichia coli in presence of zn(ii), cd(ii), or cu, and characterised by electrosp ... | 2017 | 28718783 |