Publications

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distribution and prevalence of cytauxzoon felis in bobcats (lynx rufus), the natural reservoir, and other wild felids in thirteen states.cytauxzoon felis, a protozoan parasite of wild and domestic felids, is the causative agent of cytauxzoonosis in domestic and some exotic felids in the united states. the bobcat (lynx rufus) is the natural reservoir for this parasite, but other felids such as florida panthers (puma concolor coryii) and domestic cats may maintain long-term parasitemias and serve as reservoirs. experimentally, two tick species, dermacentor variabilis and amblyomma americanum, have demonstrated the ability to transm ...201021071149
seroprevalence of toxoplasma gondii in american free-ranging or captive pumas (felis concolor) and bobcats (lynx rufus).toxoplasma gondii is a major zoonotic agent infecting a wide range of mammals, including wild felids. like domestic cats, wild felids are involved in the complete infective cycle of t. gondii, as they can host in their gastrointestinal tract sexually mature parasites and shed infective oocysts in their feces. in order to evaluate the importance of this wildlife reservoir, 438 serum samples collected between 1984 and 1999 from 438 pumas (felis concolor) and from 58 bobcats (lynx rufus) from north ...200415019138
habitat barriers limit gene flow and illuminate historical events in a wide-ranging carnivore, the american puma.we examined the effects of habitat discontinuities on gene flow among puma (puma concolor) populations across the southwestern usa. using 16 microsatellite loci, we genotyped 540 pumas sampled throughout the states of utah, colorado, arizona, and new mexico, where a high degree of habitat heterogeneity provides for a wide range of connective habitat configurations between subpopulations. we investigated genetic structuring using complementary individual- and population-based analyses, the latter ...200515910319
models of regional habitat quality and connectivity for pumas (puma concolor) in the southwestern united states.the impact of landscape changes on the quality and connectivity of habitats for multiple wildlife species is of global conservation concern. in the southwestern united states, pumas (puma concolor) are a well distributed and wide-ranging large carnivore that are sensitive to loss of habitat and to the disruption of pathways that connect their populations. we used an expert-based approach to define and derive variables hypothesized to influence the quality, location, and permeability of habitat f ...201324367495
novel gammaherpesviruses in north american domestic cats, bobcats, and pumas: identification, prevalence, and risk factors.gammaherpesviruses (ghvs) are a diverse and rapidly expanding group of viruses associated with a variety of disease conditions in humans and animals. to identify felid ghvs, we screened domestic cat (felis catus), bobcat (lynx rufus), and puma (puma concolor) blood cell dna samples from california, colorado, and florida using a degenerate pan-ghv pcr. additional pan-ghv and long-distance pcrs were used to sequence a contiguous 3.4-kb region of each putative virus species, including partial glyco ...201424453374
identification of novel gammaherpesviruses in ocelots (leopardus pardalis) and bobcats (lynx rufus) in panama and colorado, usa.gammaherpesviruses (ghvs) have been identified in many species and are often associated with disease. recently, we characterized three novel felid ghvs in domestic cats (felis catus), bobcats (lynx rufus), and pumas (puma concolor). we investigated whether free-ranging ocelots (leopardus pardalis) and bobcats are infected with additional ghvs. we screened dna samples from ocelots on barro colorado island, panama, and bobcats in western colorado, us, by using a degenerate nested pcr that targets ...201526280877
characterization of regionally associated feline immunodeficiency virus (fiv) in bobcats (lynx rufus).feline immunodeficiency virus (fiv) classically infects felid species with highly divergent species-specific fivs. however, recent studies have detected an fiv strain infecting both bobcats (lynx rufus) and pumas (puma concolor) in california and florida. to further investigate this observation, we evaluated fiv from bobcats in florida (n=25) and colorado (n=80) between 2008 and 2011. partial viral sequences from five florida bobcats cluster with previously published sequences from florida panth ...201323778629
pathogen exposure varies widely among sympatric populations of wild and domestic felids across the united states.understanding how landscape, host, and pathogen traits contribute to disease exposure requires systematic evaluations of pathogens within and among host species and geographic regions. the relative importance of these attributes is critical for management of wildlife and mitigating domestic animal and human disease, particularly given rapid ecological changes, such as urbanization. we screened > 1000 samples from sympatric populations of puma (puma concolor), bobcat (lynx rufus), and domestic ca ...201627209780
bartonella rochalimae and b. vinsonii subsp. berkhoffii in wild carnivores from colorado, usa.spleen samples from 292 wild carnivores from colorado, us were screened for bartonella infection. bartonella dna was detected in coyotes ( canis latrans ) (28%), striped skunks ( mephitis mephitis ) (23%), red foxes ( vulpes vulpes ) (27%), and raccoons ( procyon lotor ) (8%) but not in black bears ( ursus americanus ), gray foxes ( urocyon cinereoargenteus ), and mountain lions ( puma concolor ). two bartonella species, b. vinsonii subsp. berkhoffii and b. rochalimae, were identified. all 10 in ...201627529290
mountain lions prey selectively on prion-infected mule deer.the possibility that predators choose prey selectively based on age or condition has been suggested but rarely tested. we examined whether mountain lions (puma concolor) selectively prey upon mule deer (odocoileus hemionus) infected with chronic wasting disease, a prion disease. we located kill sites of mountain lions in the northern front range of colorado, usa, and compared disease prevalence among lion-killed adult (> or =2 years old) deer with prevalence among sympatric deer taken by hunters ...201019864271
occurrence of puma lentivirus infection in cougars from washington.puma lentivirus (plv) antibodies were detected in 13 (25%) of 52 serum samples obtained from cougars (felis concolor) collected by hunters. the serum samples were collected from november 1993 through january 1994 from four specific regions throughout the state of washington (usa), and included the olympic mountains, the cascade mountains, the blue mountains, and the selkirk mountains. more (38%) seropositive cougar samples originated from the cascade mountains than from any other site. the overa ...19979131566
assessing timing and causes of neonatal lamb losses in a bighorn sheep ovis canadensis canadensis herd via use of vaginal implant transmitters.we evaluated the use of vaginal implant transmitters (vits) as a means of detecting, capturing, and radio collaring rocky mountain bighorn sheep ( ovis canadensis canadensis) lambs to estimate survival and to facilitate carcass recovery to assess causes of mortality. we focused on one of several bighorn herds in colorado, usa, suffering from depressed recruitment that was not preceded by a classic all-age die-off. we captured, radio-collared, diagnosed pregnancy by ultrasound examination, and in ...201728192042
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