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characterization of aquabirnaviruses from flounder pseudopleuronectes americanus and mummichog fundulus heteroclitus in the chesapeake bay, virginia, usa.viruses were isolated in cell culture from tissue homogenates of flounder pseudopleuronectes americanus and mummichog fundulus heteroclitus in the chesapeake bay, virginia, usa. neutralization and immunofluorescence tests with aquabirnavirus (west buxton strain)-specific polyclonal antisera indicated that both viruses were aquabirnaviruses belonging to serogroup a, the most common aquabirnavirus serogroup in the united states. this was confirmed by rt-pcr, with primers targeting the vp3 and vp2 ...200314667031
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