| isolation of viral haemorrhagic septicaemia virus from muskellunge, esox masquinongy (mitchill), in lake st clair, michigan, usa reveals a new sublineage of the north american genotype. | viral haemorrhagic septicaemia virus (vhsv) was isolated from muskellunge, esox masquinongy (mitchill), caught from the nw portion of lake st clair, michigan, usa in 2003. affected fish exhibited congestion of internal organs; the inner wall of the swim bladder was thickened and contained numerous budding, fluid-filled vesicles. a virus was isolated using fish cell lines inoculated with a homogenate of kidney and spleen tissues from affected fish. focal areas of cell rounding and granulation app ... | 2006 | 17026670 |
| heterogeneity in levels of serum neutralizing antibodies against viral hemorrhagic septicemia virus genotype ivb among fish species in lake st. clair, michigan, usa. | the presence of neutralizing antibodies against viral hemorrhagic septicemia virus (vhsv-ivb) was investigated in sera of 13 fish species collected from lake st. clair, michigan, usa, a vhsv-endemic water body. we tested 297 sera collected may 2004-june of 2010, using a complement-dependent 50% plaque neutralization test (50% pnt). neutralizing antibodies were detected in 23% (67/297) of the samples. the highest overall antibody prevalence (85%, 34/40) and mean positive antibody titer (12,113 ± ... | 2012 | 22493115 |