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frequency of sarcocystis spp in detroit, metropolitan area, michigan.a survey of sarcocystis spp in feral animals and in domesticated animals slaughtered in detroit, mi, was undertaken during a 6-month period, april to september, 1973, using histologic and digestion diagnostic techniques. results indicated 75.3% of 789 sheep and 10.8% of 306 lambs were infected. infection in young and adult sheep was low in april (0% of 78 lambs and 6.7% of 62 adult sheep), but increased in july (24% of 90 lambs and 94.1% of 201 adult sheep). high frequency of sarcocystis in summ ...1975803806
mycobacterium bovis in coyotes from michigan.during a survey for tuberculosis in wild carnivores and omnivores, mycobacterium bovis was cultured from pooled lymph nodes of three adult female coyotes (canis latrans) harvested by hunters in michigan (usa). no gross or histologic lesions suggestive of tuberculosis were seen in these animals. one coyote was taken from montmorency county and two coyotes from alcona county located in the north-eastern portion of michigan's lower peninsula where free-ranging white-tailed deer (odocoileus virginia ...19989706576
the seroprevalence of antibodies to sarcocystis neurona in michigan equids.a cross-sectional study of serum antibodies to sarcocystis neurona (the etiologic agent of equine protozoal myeloencephalitis, epm) was performed on michigan equids. our objectives were to determine the seroprevalence of antibodies to s. neurona in michigan equids and to identify specific risk factors for seropositivity. a random, weighted sample of michigan horse farms (stratified by the state's opossum (didelphis virginiana) population and the number of equids on each operation) was selected. ...200111154784
bovine tuberculosis in free-ranging carnivores from michigan.during a survey of carnivores and omnivores for bovine tuberculosis conducted in michigan (usa) since 1996, mycobacterium bovis was cultured from lymph nodes pooled from six coyotes (canis latrans) (four adult female, two adult male), two adult male raccoons (procyon lotor), one adult male red fox (vulpes vulpes), and one 1.5-yr-old male black bear (ursus americanus). one adult, male bobcat (felis rufus) with histologic lesions suggestive of tuberculosis was negative on culture but positive for ...200111272505
experimental inoculation of north american opossums (didelphis virginiana) with mycobacterium bovis.eight north american opossums (didelphis virginiana) were inoculated with 1 x 10(5) colony forming units of mycobacterium bovis to investigate their potential as reservoir hosts for bovine tuberculosis in michigan. four animals received this dose orally and four were inoculated intramuscularly (i.m.). in each group, two animals were euthanized 1 mo postinoculation (pi) and two at 2 mo pi. four control animals were housed separately and sacrificed in the same manner as those inoculated. one of fo ...200212038125
prevalence and tissue distribution of besnoitia darlingi cysts in the virginia opossum (didelphis virginiana) in michigan.specimens of virginia opossums (didelphis virginiana) in michigan were examined over 1 year to document the presence of besnoitia darlingi cysts. cyst morphology, prevalence, seasonal variation, and tissue sites of isolation were studied. histology and ultrastructural features of the detected cysts and bradyzoites were consistent with b. darlingi. in the opossums, b. darlingi had intracellular tissue cysts. tissue cysts had a mean diameter of 560 microm and were separated from the host tissue by ...200312944045
observations on besnoitiosis in virginia opossum (didelphis virginiana) from michigan, usa.besnoitia tissue cysts were found in five naturally-infected adult opossums (didelphis virginiana) from michigan. details of the microscopy, histopathology, ultra-structure, and genetic features of the cysts were studied to identify their species-specific traits. the materials were differentiated phenotypically from cysts of other besnoitia spp. by difference in size, pattern of tissue distribution, morphology of pellicle and nucleus, number of micronemes and rhoptries, amount of lipids and amyl ...200717580564
ultrastructure characteristics of besnoitia darlingitachyzoites brumpt, 1913 (protozoa: sarcocystidae) of the michigan strain mibd1.tachyzoites of besnoitia darlingi brumpt, 1913 were redescribed based on new materials isolated from virginia opossums (didelphis virginiana, kerr) from michigan, u.s.a. tachyzoites of the mibd1 strain were propagated in bovine turbinate cell culture for more than two years. a comparison with previously described tachyzoites of the b. darlingi op1 strain from mississippi, usa revealed some morphological differences despite the remarkable genetic homogeneity between the two b. darlingi strains. m ...200718383798
epizootiologic survey of mycobacterium bovis in wildlife and farm environments in northern michigan.bovine tuberculosis (bovine tb), caused by mycobacterium bovis, has reemerged in northern michigan, usa, with detections in white-tailed deer (odocoileus virginianus) in 1994 and in cattle in 1998. since then, significant efforts have been directed toward reducing deer densities in the area in the hopes of reducing the bovine tb prevalence rate in deer and eliminating spillover of the disease into cattle. despite the success of the efforts to reduce deer densities, additional cattle herds have b ...201020688630
surveillance and movements of virginia opossum (didelphis virginiana) in the bovine tuberculosis region of michigan.wildlife reservoir hosts of bovine tuberculosis (btb) include eurasian badgers (meles meles) and brushtail possum (trichosurus vulpecula) in the uk and new zealand, respectively. similar species warrant further investigation in the northern lower peninsula of michigan, usa due to the continued presence of btb on cattle farms. most research in michigan, usa has focused on interactions between white-tailed deer (odocoileus virginianus) and cattle (bos taurus) for the transmission of the infectious ...201323531427
prevalence of and risk factors associated with the presence of sarcocystis neurona sporocysts in opossum (didelphis virginiana) from michigan: a retrospective study.from april 1996 to december 2002 the prevalence of sarcocystis neurona sporocysts in north american opossum (didelphis virginiana) in southern michigan was estimated. sporocysts of s. neurona were found in intestinal scrapings from 31 (15%) of 206 examined opossum. the frequency of infection was higher in adult animals (26/206; 12.6%) and females (19/206; 9.2%) than in juveniles (5/206; 2.4%) and males (12/206; 5.8%). also, prevalence of s. neurona sporocysts in opossums in relation to factors s ...200415482884
evaluating wildlife-cattle contact rates to improve the understanding of dynamics of bovine tuberculosis transmission in michigan, usa.direct and indirect contacts among individuals drive transmission of infectious disease. when multiple interacting species are susceptible to the same pathogen, risk assessment must include all potential host species. bovine tuberculosis (btb) is an example of a disease that can be transmitted among several wildlife species and to cattle, although the potential role of several wildlife species in spillback to cattle remains unclear. to better understand the complex network of contacts and factor ...201627931926
prevalence of sarcocystis species sporocysts in northern virginia opossums (didelphis virginiana).a total of 206 virginia opossums ( didelphis virginiana) collected from the mid-michigan region, united states, during a period extending from 1996 to 2002 were sampled for the presence of sarcocystis spp sporocysts. all isolates were phenotypically identified as sarcocystis spp and genotyped to the species level by pcr-based techniques. the overall prevalence of sarcocystis spp in opossums was 18% (37/206). the prevalence of sarcocystis spp differed significantly with age ( p<0.001) and adult o ...200415205944
sarcocystis inghami n. sp. (sporozoa: sarcocystidae) from the skeletal muscles of the virginia opossum didelphis virginiana in michigan.this report describes the newly identified sarcocystis inghami n. sp. from the skeletal muscles of opossums (mammalia: didelphidae) that were collected from south central michigan (42 degrees 43'-42 degrees 79'n, 84 degrees 18'-84 degrees 86'w), usa. the new species is distinguished from all species described from north and south american opossums by the distinctive morphology of the villar protrusions on the cyst wall. sarcocysts of s. inghami are microscopic, up to 700 microm long and 110 micr ...200312975625
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