Publications

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using faecal dna sampling and gis to monitor hybridization between red wolves (canis rufus) and coyotes (canis latrans).the us fish and wildlife service's (usfws) red wolf recovery program recognizes hybridization with coyotes as the primary threat to red wolf recovery. efforts to curb or stop hybridization are hampered in two ways. first, hybrid individuals are difficult to identify based solely on morphology. second, managers need to effectively search 6000 km(2) for the presence of coyotes and hybrids. we develop a noninvasive method to screen large geographical areas for coyotes and hybrids with maternal coyo ...200312859637
assessing the prevalence of hybridization between sympatric canis species surrounding the red wolf (canis rufus) recovery area in north carolina.predicting spatial patterns of hybridization is important for evolutionary and conservation biology yet are hampered by poor understanding of how hybridizing species can interact. this is especially pertinent in contact zones where hybridizing populations are sympatric. in this study, we examined the extent of red wolf (canis rufus) colonization and introgression where the species contacts a coyote (c. latrans) population in north carolina, usa. we surveyed 22 000 km(2) in the winter of 2008 for ...201121486372
canine schistosomiasis in north america: an underdiagnosed disease with an expanding distribution.heterobilharzia americana, a digenean trematode in the family schistosomatidae, is the etiologic agent of canine schistosomiasis in the southeastern united states.1 a few cases of canine schistosomiasis have been reported in florida, louisiana, north carolina, texas, and, recently, kansas.1-6 the natural definitive host for the fluke is the raccoon1; however, infections have been detected in nutrias, bobcats, mountain lions, opossums, white-tailed deer, swamp rabbits, armadillos, coyotes, red wo ...201020473851
locating hybrid individuals in the red wolf (canis rufus) experimental population area using a spatially targeted sampling strategy and faecal dna genotyping.hybridization with coyotes (canis latrans) continues to threaten the recovery of endangered red wolves (canis rufus) in north carolina and requires the development of new strategies to detect and remove coyotes and hybrids. here, we combine a spatially targeted faecal collection strategy with a previously published reference genotype data filtering method and a genetic test for coyote ancestry to screen portions of the red wolf experimental population area for the presence of nonred wolf canids. ...200717444895
captive breeding and the reintroduction of mexican and red wolves.mexican and red wolves were both faced with extinction in the wild until captive populations were established more than two decades ago. these captive populations have been successfully managed genetically to minimize mean kinship and retain genetic variation. descendants of these animals were subsequently used to start reintroduced populations, which now number about 40-50 mexican wolves in arizona and new mexico and about 100 red wolves in north carolina. the original captive mexican wolf popu ...200818173506
a gene expression signature of confinement in peripheral blood of red wolves (canis rufus).the stresses that animals experience as a result of modification of their ecological circumstances induce physiological changes that leave a signature in profiles of gene expression. we illustrate this concept in a comparison of free range and confined north american red wolves (canis rufus). transcription profiling of peripheral blood samples from 13 red wolf individuals in the alligator river region of north carolina revealed a strong signal of differentiation. four hundred eighty-two out of 2 ...200818466232
evaluating the ability of bayesian clustering methods to detect hybridization and introgression using an empirical red wolf data set.bayesian clustering methods have emerged as a popular tool for assessing hybridization using genetic markers. simulation studies have shown these methods perform well under certain conditions; however, these methods have not been evaluated using empirical data sets with individuals of known ancestry. we evaluated the performance of two clustering programs, baps and structure, with genetic data from a reintroduced red wolf (canis rufus) population in north carolina, usa. red wolves hybridize with ...201323163531
inbreeding and inbreeding depression in endangered red wolves (canis rufus).in natural populations, the expression and severity of inbreeding depression can vary widely across taxa. describing processes that influence the extent of inbreeding and inbreeding depression aid in our understanding of the evolutionary history of mating systems such as cooperative breeding and nonrandom mate selection. such findings also help shape wildlife conservation theory because inbreeding depression reduces the viability of small populations. we evaluated the extent of inbreeding and in ...201425060763
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