| nucleotide sequence and deduced amino acid sequence of the nonstructural proteins of dengue type 3 virus, bangkok genotype. | the nucleotide sequence of the nonstructural protein gene (1,610 bases) of dengue 3 virus (bangkok genotype; ch53489 isolated in 1973) has been determined in both forward and reverse directions. the pcr based cycle sequencing technic by the enzymatic method of sanger et al using a sequencing primer 5'-end labeled with gamma-32p-atp was the method of our choice for sequence analysis. two cdna templates were prepared by rt-pcr technique starting from the nucleotides 6,306-6,969 and 6,925-7,915 of ... | 1998 | 9886128 |