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molecular characterization of anisakis larvae from fish caught off sardinia.abstract anisakis spp. larvae are parasitic, and potentially zoonotic, nematodes transmitted by marine fish and cephalopods, which are the main intermediate hosts of the third larval stage. the accidental consumption of infected raw or poorly cooked fish may cause gastroenteric diseases and allergies in humans. the aim of the present study was to use pcr-rflp to define the occurrence, species variability, and host preferences of anisakis spp. larvae in fish caught off the coast of sardinia. necr ...201121501045
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