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the use of cyprinodont fish, aphanius fasciatus, as a sentinel organism to detect complex genotoxic mixtures in the coastal lagoon ecosystem.in the present work we aimed to standardise the alkaline comet assay with erythrocytes of the cyprinodont, mediterranean killifish, aphanius fasciatus. the aims of the study were to explore the suitability of this fish to assess biomarkers of genotoxic effects and as a sentinel organism to detect complex genotoxic mixtures in coastal lagoon ecosystems. following proper optimisation, the application and effectiveness of the comet assay in erythrocytes of a. fasciatus were tested by measuring the ...201222222993
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