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survey of leptospira spp in pampas deer (ozotoceros bezoarticus) in the pantanal wetlands of the state of mato grosso do sul, brazil by serology and polymerase chain reaction.this work reports a survey of leptospira spp in pampas deer (ozotoceros bezoarticus) in the pantanal wetlands of the state of mato grosso do sul, brazil by serology and polymerase chain reaction (pcr). seventy pampas deer were captured in the dry season and surveyed using pcr, microscopic agglutination test (mat) (n = 51) and by both techniques (n = 47). pcr detected infections in two pampas deer and mat detected infections in three. through sequencing and phylogenetic analyses, the pcr-amplifie ...201122012234
molecular detection and identification of hemoparasites in pampas deer (ozotoceros bezoarticus linnaeus, 1758) from the pantanal brazil.hemoparasites were surveyed in 60 free-living pampas deer ozotoceros bezoarticus from the central area of the pantanal, known as nhecolândia, state of mato grosso do sul, brazil, through the analysis of nested pcr assays and nucleotide sequencing. blood samples were tested for babesia/theileria, anaplasma spp., and trypanosoma spp. using npcr assays and sequencing of the 18s rrna, msp4, its, and cathepsin l genes. the identity of each sequence was confirmed by comparison with sequences from genb ...201323567028
serosurvey for antibodies against brucella abortus and leptospira interrogans in pampas deer from brazil.a survey for antibodies against brucella abortus, and leptospira interrogans was conducted on 17 pampas deer (ozotocerus bezoarticus) from pantanal matogrossense (state of mato grosso do sul, brazil) and on 24 pampas deer from parque nacional de emas (state of goiás, brazil). antibodies against b. abortus were detected by plate agglutination, rose bengal, and complement fixation tests; antibodies against leptospira interrogans were detected by the microscopic agglutination test. all sera were ne ...199910073359
environmental effect on the occurrence of anti-neospora caninum antibodies in pampas-deer (ozotoceros bezoarticus).sera obtained from pampas-deer (ozotoceros bezoarticus) captured in two different brazilian environments were analyzed for the presence of anti-neospora caninum antibodies by the indirect fluorescent antibody test (ifat). samples were collected from 23 animals from a savanna area in the national park of emas, in the state of goiás, brazil. this area is surrounded by cultivated lands and allows very little contact between wild and domestic animals. another batch of samples was collected from 16 a ...200516112811
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