| sensitivity of the polymerase chain reaction for the diagnosis of cutaneous leishmaniasis due to leishmania (viannia) guyanensis. | the sensitivity of the polymerase chain reaction (pcr) in 35 consecutive outpatients with cutaneous leishmaniasis caused by leishmania (viannia) guyanensis was evaluated using, as gold standard, the in vitro isolation of the parasite through culture of aspirates of the cutaneous ulcers. all isolates were identified using electrophoretic enzyme analysis. patients were mainly young males with recent onset disease without prior specific treatment. pcr was performed using dna extracted from fresh fr ... | 2001 | 11412806 |
| trypanosoma (megatrypanum) lainsoni n. sp. from mesomys hispidus (rodentia: echimyidae) in brazil: trypomastigotes described from experimentally infected laboratory mice. | we report the detection, isolation and description of trypanosoma (megatrypanum) lainsoni n. sp. from a caviomorph rodent, mesomys hispidus (rodentia: echimyidae), obtained in the rio negro region of the state of amazonas, in northern brazil. laboratory-bred white mice (mus musculus) and rats (rattus rattus) were inoculated with large numbers of culture forms by intraperitoneal route, and trypomastigotes appeared in their blood 3-8 days post-inoculation. one single epimastigote was also found in ... | 2013 | 24309069 |