| detection of australian gill-associated virus (gav) and lymphoid organ virus (lov) of penaeus monodon by rt-nested pcr. | a highly sensitive test based on reverse transcription followed by nested polymerase chain reaction (rt-npcr) was developed to detect the australian yellow-head-like viruses, gill-associated virus (gav) and lymphoid organ virus (lov) of penaeus monodon. the rt-npcr detected viral rna in as little as 10 fg lymphoid organ total rna isolated from gav-infected p. monodon. amplification of serial dilutions of a gav cdna clone showed that the nested pcr was sufficiently sensitive to detect a single ge ... | 2000 | 10768283 |