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axoneme-dependent tubulin modifications in singlet microtubules of the drosophila sperm tail.drosophila melanogaster sperm tubulins are posttranslationally glutamylated and glycylated. we show here that axonemes are the substrate for these tubulin c-terminal modifications. axoneme architecture is required, but full length, motile axonemes are not necessary. tubulin glutamylation occurs during or shortly after assembly into the axoneme; only glutamylated tubulins are glycylated. tubulins in other testis microtubules are not modified. only a small subset of total drosophila sperm axoneme ...200818205200
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