| diminazene aceturate in the treatment of experimentally induced trypanosoma brucei brucei infection in domestic chickens. | | 1982 | 7179701 |
| wildtype and engineered monomeric triosephosphate isomerase from trypanosoma brucei: partitioning of reaction intermediates in d(2)o and activation by phosphite dianion. | product yields for the reactions of (r)-glyceraldehyde 3-phosphate (gap) in d(2)o at pd 7.9 catalyzed by wildtype triosephosphate isomerase from trypanosoma brucei brucei (tbb tim) and a monomeric variant (monotim) of this wildtype enzyme were determined by (1)h nmr spectroscopy and were compared with the yields determined in earlier work for the reactions catalyzed by tim from rabbit and chicken muscle [ o'donoghue , a. c. , amyes , t. l. , and richard , j. p. ( 2005 ) , biochemistry 44 , 2610 ... | 2011 | 21553855 |
| production of anti-peptide antibodies against trypanopain-tb from trypanosoma brucei brucei: effects of antibodies on enzyme activity against z-phe-arg-amc. | anti-peptide antibodies were produced against the cysteine proteinase trypanopain-tb from trypanosoma brucei brucei and the effects of these antibodies on enzyme activity against carboxybenzoyl (z)-phe-arg-aminomethylcoumarin (amc) investigated. a peptide was synthesised corresponding to a region of the trypanopain-tb active site around the active site histidine so that the resulting anti-peptide antibodies specifically targeted the active site of the enzyme. such antibodies were considered more ... | 1997 | 9228560 |
| massive increase in splenic germinal centres of chickens experimentally-infected with trypanosoma brucei brucei. | domestic chickens infected with trypanosoma brucei brucei developed a latent parasitaemia which lasted for one year. six distinct variable antigen types were isolated. spleens from infected birds were studied histologically at different stages of the infection. trypanosome infection produced a progressive increase in the number of germinal centres during the early stages of the infection. peak numbers of germinal centres were reached in the spleen at day 84 after which the numbers were maintaine ... | 1983 | 6685396 |
| structure determination of the glycosomal triosephosphate isomerase from trypanosoma brucei brucei at 2.4 a resolution. | the three-dimensional crystal structure of the enzyme triosephosphate isomerase from the unicellular tropical blood parasite trypanosoma brucei brucei has been determined at 2.4 a resolution. this triosephosphate isomerase is sequestered in the glycosome, a unique trypanosomal microbody of vital importance for the energy-generating machinery of the trypanosome. the crystals contain one dimer per asymmetric unit. the structure could be solved by the method of molecular replacement, using the refi ... | 1987 | 3430602 |
| kinetic properties of triose-phosphate isomerase from trypanosoma brucei brucei. a comparison with the rabbit muscle and yeast enzymes. | the kinetic properties of trypanosoma brucei brucei triose-phosphate isomerase are compared with those of the commercially available rabbit muscle and yeast enzymes and with published data on the chicken muscle enzyme. with glyceraldehyde 3-phosphate as substrate km = 0.25 +/- 0.05 mm and kcat = 3.7 x 10(5) min-1. with dihydroxyacetone phosphate as substrate km = 1.2 +/- 0.1 mm and kcat = 6.5 x 10(4) min-1. the ph dependence of km and vmax at 0.1 m ionic strength is in agreement with the results ... | 1987 | 3311744 |
| characterization of an endopeptidase of trypanosoma brucei brucei. | a soluble 80-kda endopeptidase has been isolated from trypanosoma brucei brucei. the enzyme, which has a pi 5.1, is optimally active at about ph 8.2 and has apparent pka values of 6.0 and greater than or equal to 10. it is inhibited by the serine protease inhibitor diisopropylfluorophosphate and by the serine protease mechanism-based inhibitor 3,4-dichloroisocoumarin. unexpectedly, the enzyme is inhibited by the cysteine protease inhibitor benzyloxycarbonyl-leu-lys-chn2 but not by the related di ... | 1992 | 1731636 |
| the significance of human serum sensitivity in the context of t.b. rhodesiense sleeping sickness epidemiology and control. | earlier this century the postulate that trypanosoma brucei brucei and t.b. rhodesiense had a common identity and that human infectability was linked with resistance to normal human serum (nhs) in vitro, were both finally refuted in the classical tinde experiment. interest in serum sensitivity was reawakened with the advent of the biit in 1970 and the studies that followed demonstrated the presence of both human-serum-resistant (hsr) and sensitive (hss) variant antigen types, within the surface a ... | 1992 | 1644047 |
| enzyme architecture: remarkably similar transition states for triosephosphate isomerase-catalyzed reactions of the whole substrate and the substrate in pieces. | values of (k(cat)/k(m))gap for triosephosphate isomerase-catalyzed reactions of (r)-glyceraldehyde 3-phosphate and k(cat)/k(hpi)k(ga) for reactions of the substrate pieces glycolaldehyde and hpo3(2-) have been determined for wild-type and the following tim mutants: i172v, i172a, l232a, and p168a (tim from trypanosoma brucei brucei); a 208-tgag for 208-yggs loop 7 replacement mutant (l7rm, tim from chicken muscle); and, y208t, y208s, y208a, y208f and s211a (yeast tim). a superb linear logarithmic ... | 2014 | 24588650 |