virulence of an emerging pathogenic lineage of vibrio nigripulchritudo is dependent on two plasmids. | vibrioses are the predominant bacterial infections in marine shrimp farms. vibrio nigripulchritudo is an emerging pathogen of the cultured shrimp litopenaeus stylirostris in new caledonia and other regions in the indo-pacific. the molecular determinants of v. nigripulchritudo pathogenicity are unknown; however, molecular epidemiological studies have revealed that recent pathogenic v. nigripulchritudo isolates from new caledonia all cluster into a monophyletic clade and contain a small plasmid, p ... | 2011 | 20825454 |
metal and metalloid bioaccumulation in the pacific blue shrimp litopenaeus stylirostris (stimpson) from new caledonia: laboratory and field studies. | the present work aimed at better understanding metal and metalloid bioaccumulation in the edible pacific blue shrimp litopenaeus stylirostris, using both laboratory and field approaches. in the laboratory, the bioaccumulation kinetics of ag, cd, co, cr, and zn have been investigated in shrimp exposed via seawater and food, using the corresponding gamma-emitting radiotracers ((110 m)ag, (109)cd, (57)co, (51)cr, and (65)zn) and highly sensitive nuclear detection techniques. results showed that hep ... | 2010 | 20637480 |
quantification of vibrio penaeicida, the etiological agent of syndrome 93 in new caledonian shrimp, by real-time pcr using sybr green i chemistry. | shrimp farming is a small but growing industry in new caledonia. since 1993, "syndrome 93" has been affecting new caledonian shrimp farming industry every cold season, causing severe epizootic mortalities in grow-out ponds and significant losses. highly pathogenic strains of vibrio penaeicida are considered the etiological agent of the disease in litopenaeus stylirostris. on one hand, studies demonstrated that healthy shrimp may carry v. penaeicida for weeks with a high overall prevalence, regar ... | 2006 | 16616385 |
molecular epidemiology of vibrio nigripulchritudo, a pathogen of cultured penaeid shrimp (litopenaeus stylirostris) in new caledonia. | a collection of 57 isolates of vibrio nigripulchritudo from either diseased or healthy shrimp and from shrimp farms environment was studied in order to gain a better understanding of the epidemiology of this pathogen, notably isolated from two distinct shrimp disease complexes. molecular typing using two different techniques, arbitrarily primed pcr (ap-pcr) and multi-locus sequence typing (mlst), studied together with experimental pathology data allowed a relevant epidemiological insight into th ... | 2006 | 16413158 |
rapid and sensitive pcr detection of vibrio penaeicida, the putative etiological agent of syndrome 93 in new caledonia. | experimental infections of penaeus (litopenaeus) stylirostris were performed with a vibrio penaeicida strain (am101) isolated in new caledonia from syndrome 93 diseased shrimp. cumulative mortalities resulting from intramuscular injection or immersion of shrimp in bacterial suspensions demonstrated high virulence for this bacterial strain and suggested that v. penaeicida could be the etiological agent of syndrome 93. the median lethal dose (ld50) for am101 was 1.3 x 10(4) cfu (colony forming uni ... | 2000 | 10782344 |
correlation between detection of a plasmid and high-level virulence of vibrio nigripulchritudo, a pathogen of the shrimp litopenaeus stylirostris. | vibrio nigripulchritudo, the etiological agent of litopenaeus stylirostris summer syndrome, is responsible for mass mortalities of shrimp in new caledonia. epidemiological studies led to the suggestion that this disease is caused by an emergent group of pathogenic strains. genomic subtractive hybridization was carried out between two isolates exhibiting low and high virulence. our subtraction library was constituted of 521 specific fragments; 55 of these were detected in all virulent isolates fr ... | 2008 | 18359828 |