| the effects of tertiary treated municipal wastewater on fish communities of a small river tributary in southern ontario, canada. | fish community changes associated with a tertiary treated municipal wastewater effluent outfall in the speed river, ontario, canada, were evaluated at nine sites over two seasons (2008) using standardized electrofishing. habitat evaluations were conducted to ensure that the riffle sites selected were physically similar. the fish community was dominated by several species of darters that differed in their response to the effluent outfall. there was a significant decrease in greenside darter (ethe ... | 2011 | 21489666 |
| how does reference site selection influence interpretation of omics data?: evaluating liver transcriptome responses in male rainbow darter (etheostoma caeruleum) across an urban environment. | studies quantifying the influence of reference site selection on transcriptomic profiles in aquatic organisms exposed to complex mixtures are lacking in the literature, despite the significant implications of such research for the interpretation of omics data sets. we measured hepatic transcriptomic responses in fish across an urban environment in the central grand river watershed (ontario, canada). adult male rainbow darter (rbd) (etheostoma caeruleum) were collected from nine sites at varying ... | 2017 | 28489360 |
| whole organism responses and intersex severity in rainbow darter (etheostoma caeruleum) following exposures to municipal wastewater in the grand river basin, on, canada. part a. | municipal wastewater effluents (mwwes) contain anthropogenic substances that can exhibit endocrine-disrupting activity. these complex mixtures have been observed to exert adverse effects on fish. rainbow darter (etheostoma caeruleum, rbd) is a small benthic fish that is widespread throughout the grand river, ontario, canada, and has been previously shown to be adversely affected by mwwe exposure in this watershed. the objectives of this study were to quantify biological responses in this sentine ... | 2015 | 25543172 |
| severe intersex is predictive of poor fertilization success in populations of rainbow darter (etheostoma caeruleum). | municipal wastewater effluent (mwwe) contains emerging contaminants such as pharmaceuticals and personal care products (ppcps) that have estrogenic properties. ppcps are thought to be responsible for feminization of male fish in heavily urbanized areas around the globe. while many observations of feminized male fish have been made, the impact of feminization on reproductive success is not well understood. to address this lack of knowledge of the impacts of feminization, we examined the reproduct ... | 2015 | 25630044 |
| characterizing transcriptional networks in male rainbow darter (etheostoma caeruleum) that regulate testis development over a complete reproductive cycle. | intersex is a condition that has been associated with exposure to sewage effluents in male rainbow darter (etheostoma caeruleum). to better understand changes in the transcriptome that are associated with intersex, we characterized annual changes in the testis transcriptome in wild, unexposed fish. rainbow darter males were collected from the grand river (ontario, canada) in may (spawning), august (post-spawning), october (recrudescence), january (developing) and march (pre-spawning). histology ... | 2016 | 27861489 |
| returning to normal? assessing transcriptome recovery over time in male rainbow darter (etheostoma caeruleum) liver in response to wastewater-treatment plant upgrades. | the present study measured hepatic transcriptome responses in male rainbow darter (etheostoma caeruleum) exposed to 2 municipal wastewater-treatment plants (mwwtps; kitchener and waterloo) over 4 fall seasons (2011-2014) in the grand river (ontario, canada). the overall goal was to determine if upgrades at the kitchener mwwtp (in 2012) resulted in transcriptome responses indicative of improved effluent quality. the number of differentially expressed probes in fish downstream of the kitchener out ... | 2017 | 28106290 |
| an assessment of the spatial and temporal variability of biological responses to municipal wastewater effluent in rainbow darter (etheostoma caeruleum) collected along an urban gradient. | municipal wastewater effluent (mwwe) and its constituents, such as chemicals of emerging concern, pose a potential threat to the sustainability of fish populations by disrupting key endocrine functions in aquatic organisms. while studies have demonstrated changes in biological markers of exposure of aquatic organisms to groups of chemicals of emerging concern, the variability of these markers over time has not been sufficiently described in wild fish species. the aim of this study was to assess ... | 2016 | 27776151 |
| molecular pathways associated with the intersex condition in rainbow darter (etheostoma caeruleum) following exposures to municipal wastewater in the grand river basin, on, canada. part b. | rainbow darter (etheostoma caeruleum; rbd) is a small benthic fish found in north america. this species is sensitive to sewage effluent, and intersex is found in up to 80% of males in near-field areas in the grand river, ontario, canada. to learn more about the molecular signaling cascades associated with intersex, a developed customized oligonucleotide microarray (4×180 k) using next generation sequencing was developed to characterize the transcriptome in the gonad of male and female rbd. gene ... | 2015 | 25542366 |
| occurrence and degree of intersex (testis-ova) in darters (etheostoma spp.) across an urban gradient in the grand river, ontario, canada. | the variability and extent of the intersex condition (oocytes in testes, or testis-ova) was documented in fish along an urban gradient in the grand river, ontario, canada, that included major wastewater treatment plant outfalls. a method for rapid enumeration of testis-ova was developed and applied that increased the capacity to quantify intersex prevalence and severity. male rainbow darters (etheostoma caeruleum) sampled downstream of the first major wastewater outfall (waterloo) had a signific ... | 2013 | 23633427 |