| purifying the hedgehog protein and its variants. | the purification of recombinant versions of the n-terminal signaling fragment of sonic hedgehog (shhn) from e. coli, hi-5 insect cells, yeast, and mammalian cell sources reveals diverse post-translational modifications that affect the potency of the purified protein. modifications to the n-terminal cysteine with fatty acyl groups results in significant increases in potency, up to 100-fold, when compared with the unmodified protein. proteolytic clipping at sites near the n-terminus results in ina ... | 2007 | 18025709 |
| purification and bioassay of hedgehog ligands for the study of cell death and survival. | the hedgehog (hh) family of secreted ligands-composed of sonic hedgehog (shh), indian hedgehog (ihh), and desert hedgehog (dhh)-possesses many roles during embryonic development, adult homeostasis, and cancer. the specific functions of the hh proteins are intertwined with their requirement as survival factors in hh-responsive cells. however, studies designed to dissect the anti-apoptotic role of hhs have been hindered by the lack of simple approaches to purify large quantities of recombinant lig ... | 2008 | 18603123 |