| experimental brugia malayi infections in the rhesus monkey. | twenty-eight rhesus monkeys in 3 groups were exposed to single (group i), double (group ii), and multiple (group iii) inoculations with b. malayi infective larvae. infections were monitored by microfilarial and blood counts, selected biochemical tests, ifa responses, and records of body temperature and lymphadenopathy before and/or after treatment with dec. as a whole, the highest microfilaraemia levels were observed in group ii and lowest in group iii monkeys. eosinophilia was a common occurren ... | 1977 | 411182 |
| circadian periodicity in microfilarial counts. | we propose a second-order harmonic model to describe circadian periodicity in the 24-h cycle of microfilarial counts. rhesus monkeys (macaca mulatta) were infected with brugia malayi. definite evidence of circadian periodicity was found in the microfilarial counts. the estimates of the parameters, periodicity indices and peak hours were determined. | 1990 | 2348501 |
| studies on immunization against brugia malayi infection in the rhesus monkey. | recent studies on immunity to helminthic infection have shown that some degree of protective immunity may be stimulated by inoculations of attenuated living worms or their metabolites. the present experiments were designed to observe the effects of attempted immunization in the rhesus monkey by the use of attenuated infective larvae of brugia malayi.some effect was observed in animals inoculated with larval incubate, in which microfilaraemia did develop, but at low levels and for short durations ... | 1969 | 4979899 |
| distribution of developing and mature brugia malayi in rhesus monkeys and in the jird, meriones unguiculatus, after a single infection. | | 1971 | 5003272 |
| antibody responses to experimental brugia malayi infections in patas and rhesus monkeys. | humoral antibody responses in experimental infections with brugia malayi (subperiodic strain) were compared in two primate species. erythrocebus patas and macaca mulatta. antibody responses were related to the infection protocol and the duration and magnitude of microfilaremia. patas monkeys were uniformly susceptible to infection and characteristically exhibited prolonged microfilaremia; infections in rhesus monkeys produced low and usually microfilaremia. antibody, measured by enzyme linked im ... | 1983 | 6134454 |
| brugia malayi and b. pahangi: cultivation in vitro of infective larvae to the fourth and fifth stages. | cultivation of fourth stage brugia pahangi and b. malayi larvae from infective larvae (stage 3) were obtained in culture medium rpmi 1640 supplemented with 10% human ab serum and an lcc-mk2 rhesus monkey kidney continuous cell line feeder layer. this culture system kept larvae alive in excess of 7 weeks, and served as a source for collection of the worms' secretory, excretory, and moulting antigens. | 1983 | 6832282 |
| brugia malayi: the diagnostic potential of recombinant excretory/secretory antigens. | the diagnostic potential of recombinant e/s antigens of the lymphatic filaria brugia malayi was investigated by western blot. a cdna expression library was constructed using b. malayi male adult worm mrna, and e/s recombinants were identified with a rabbit antiserum raised against e/s products collected in vitro from b. malayi male and female adult worms. two of these recombinants, bm12 and bm14l, were studied after subcloning the cdna inserts in an escherichia coli plasmid expression and purifi ... | 1994 | 8001660 |
| histopathological, lymphoscintigraphical, and immunological changes in the inguinal lymph nodes of rhesus monkeys during the early course of infection with brugia malayi. | the relationship of the early lymphatic pathophysiological alterations with those of tissue inflammatory and cellular responses in the inguinal lymph nodes of brugia malayi-infected rhesus monkeys was examined. each of five animals was inoculated subcutaneously in the right calf with 200 third stage larvae (l3) and 5 weeks later, before the onset of patency [10 to 12 weeks postinoculation (pi)], their right inguinal nodes began to show signs of enlargement, becoming most prominent between weeks ... | 1998 | 9635437 |
| diminished production of t helper 1 cytokines and lack of induction of il-2r+ t cells correlate with t-cell unresponsiveness in rhesus monkeys chronically infected with brugia malayi. | the relationship between antigen-specific responsiveness, parasitic burden, and lymphatic pathology was investigated in nine rhesus monkeys with chronic brugia malayi infections. specifically, in vitro proliferation, cytokine gene expression and production, il-2r expression on t cells, microfilaria (mf) densities, and lymphedema were evaluated. pbmc from three animals (two mf- one mf+) proliferated in response to filarial antigen (responder monkeys, rm) and cells from six animals (5 mf+; one mf- ... | 1998 | 9709033 |
| detection of serum igg antibodies specific for wolbachia surface protein in rhesus monkeys infected with brugia malayi. | the mechanism of lymphedema development in individuals with lymphatic filariasis is presently poorly understood. to investigate whether wolbachia, symbiotic bacteria living within filarial nematodes, may be involved in disease progression, wolbachia-specific immune responses were assayed in a group of brugia malayi-infected rhesus monkeys. serum igg antibodies specific for a major wolbachia surface protein (wsp) were detected in 2 of 12 infected monkeys. it is interesting that both of these monk ... | 2001 | 11443570 |
| evaluation of a multivalent vaccine against lymphatic filariasis in rhesus macaque model. | lymphatic filariasis affects 120 million people worldwide and another 1.2 billion people are at risk of acquiring the infection. chemotherapy with mass drug administration is substantially reducing the incidence of the infection. nevertheless, an effective vaccine is needed to prevent the infection and eradicate the disease. previously we reported that a multivalent fusion protein vaccine (rbmhat) composed of small heat shock proteins 12.6 (hsp12.6), abundant larval transcript-2 (alt-2) and larg ... | 2014 | 25401783 |
| improving the efficacy of a prophylactic vaccine formulation against lymphatic filariasis. | mass drug administration (mda) is the current strategy for interrupting the transmission of lymphatic filariasis (lf) infection and control of the disease in endemic areas. however, subject non-compliance has resulted in the presence of several "transmission hotspots" in the endemic regions threatening the reemergence of lf. this situation is further complicated by the fact that the drugs used in mda are not effective against adult lf worms, a major concern for the control strategy. thus, there ... | 2017 | 28828575 |