| vacuolation activity and intracellular trafficking of artb, the binding subunit of an ab5 toxin produced by salmonella enterica serovar typhi. | various salmonella enterica serovars, including s. enterica serovar typhi, encode an ab5 toxin (artab), the a subunit of which is an adp-ribosyltransferase related to the s1 subunit of pertussis toxin. however, although the a subunit is able to catalyze adp-ribosylation of host g proteins, a cytotoxic phenotype has yet to be identified for the holotoxin. here we show that its b subunit pentamer (artb) binds to receptors on the surface of vero (african green monkey kidney) cell, cho (chinese hams ... | 2017 | 28533468 |
| the long-lived nature of clostridium perfringens iota toxin in mammalian cells induces delayed apoptosis. | mono-adp ribosylation of actin by bacterial toxins, such as clostridium perfringens iota or clostridium botulinum c2 toxins, results in rapid depolymerization of actin filaments and cell rounding. here we report that treatment of african green monkey kidney (vero) cells with iota toxin resulted in delayed caspase-dependent death. unmodified actin did not reappear in toxin-treated cells, and enzyme-active toxin was detectable in the cytosol for at least 24 h. c2 toxin showed comparable, long-live ... | 2009 | 19805536 |