| passage of dengue virus type 4 vaccine candidates in fetal rhesus lung cells selects heparin-sensitive variants that result in loss of infectivity and immunogenicity in rhesus macaques. | three dengue virus type 4 (denv-4) vaccine candidates containing deletions in the 3' noncoding region were prepared by passage in dbs-frhl-2 (frhl) cells. unexpectedly, these vaccine candidates and parental denv-4 similarly passaged in the same cells failed to elicit either viremia or a virus-neutralizing antibody response. consensus sequence analysis revealed that each of the three viruses, as well as the parental denv-4 when passaged in frhl cells, rapidly acquired a single glu327-gly substitu ... | 2009 | 19656873 |
| dengue virus type 2 envelope protein displayed as recombinant phage attachment protein reveals potential cell binding sites. | a method to map the specific site on dengue virus envelope protein (e) that interacts with cells and a neutralizing antibody is developed using serially truncated dengue virus type 2 (denv-2) e displayed on m13 phages as recombinant e-g3p fusion proteins. recombinant phages displaying the truncated e consisting of amino acids 297-423 (eb2) and amino acids 379-423 (eb4) were neutralized by denv-2 patient sera and the denv-2 e-specific 3h5-1 monoclonal antibodies suggesting that the phages retaine ... | 2008 | 18669522 |
| zinc accelerates dengue virus type 2-induced apoptosis in vero cells. | dengue virus type 2 (denv-2) infection induced apoptotic cellular dna fragmentation in vero cells within 8 days of infection. the addition of high concentrations of extracellular zn(2+) but not ca(2+), mg(2+) or mn(2+) to the cell culture medium hastened the detection of apoptosis to within 4 h after infection. no apoptotic cellular dna fragmentation was detected in the cell culture treated with zn(2+) alone or infected with heat- or ultraviolet light-inactivated denv-2 in the presence of zn(2+) ... | 2002 | 12135735 |
| dengue, yellow fever, zika and chikungunya epidemic arboviruses in brazil: ultrastructural aspects. | the impact of arbovirus cocirculation in brazil is unknown. dengue virus (denv) reinfection may result in more intense viraemia or immunopathology, leading to more severe disease. the zika virus (zikv) epidemic in the americas provided pathogenicity evidence that had not been previously observed in flavivirus infections. in contrast to other flaviviruses, electron microscopy studies have shown that zikv may replicate in viroplasm-like structures. flaviviruses produce an ensemble of structurally ... | 2021 | 33566939 |
| olive baboons: a non-human primate model for testing dengue virus type 2 replication. | this study evaluated the use of a non-human primate, the olive baboon (papio anubis), as a model of dengue infection. olive baboons closely resemble humans genetically and physiologically and have been used extensively for assessing novel vaccine formulations. | 2013 | 24139794 |
| seroprevalence of zika virus in wild african green monkeys and baboons. | zika virus (zikv) has recently spread through the americas and has been associated with a range of health effects, including birth defects in children born to women infected during pregnancy. although the natural reservoir of zikv remains poorly defined, the virus was first identified in a captive "sentinel" macaque monkey in africa in 1947. however, the virus has not been reported in humans or nonhuman primates (nhps) in africa outside gabon in over a decade. here, we examine zikv infection in ... | 2017 | 28289727 |
| dengue viruses cluster antigenically but not as discrete serotypes. | the four genetically divergent dengue virus (denv) types are traditionally classified as serotypes. antigenic and genetic differences among the denv types influence disease outcome, vaccine-induced protection, epidemic magnitude, and viral evolution. we characterized antigenic diversity in the denv types by antigenic maps constructed from neutralizing antibody titers obtained from african green monkeys and after human vaccination and natural infections. genetically, geographically, and temporall ... | 0 | 26383952 |
| infection dynamics of sylvatic dengue virus in a natural primate host, the african green monkey. | the four serotypes of mosquito-borne dengue virus (denv-1, -2, -3, and -4) that circulate in humans each emerged from an enzootic, sylvatic cycle in non-human primates. herein, we present the first study of sylvatic denv infection dynamics in a primate. three african green monkeys were inoculated with 10(5) plaque-forming units (pfu) denv-2 strain pm33974 from the sylvatic cycle, and one african green monkey was inoculated with 10(5) pfu denv-2 strain new guinea c from the human cycle. all four ... | 2014 | 25092823 |
| live attenuated tetravalent dengue virus host range vaccine is immunogenic in african green monkeys following a single vaccination. | the causative agent of dengue fever, dengue virus (denv), is transmitted by mosquitoes, and as distribution of these insects has expanded, so has dengue-related disease. denv is a member of the flaviviridae family and has 4 distinct serotypes (denv-1, -2, -3, and -4). no lasting cross protection is afforded to heterologous serotypes following infection by any one of the individual serotypes. the presence of nonneutralizing antibodies to one serotype can facilitate the occurrence of more-severe d ... | 2014 | 24696467 |
| testing of novel dengue virus 2 vaccines in african green monkeys: safety, immunogenicity, and efficacy. | the immunogenicity and safety of three novel host-range vaccines containing deletions in the transmembrane domain of dengue virus serotype 2 (dv2) e glycoprotein were evaluated in african green monkeys. the shorter transmembrane domains are capable of functionally spanning an insect but not a mammalian cell membrane, resulting in production of viral mutants that have reduced infectivity in mammalian hosts but efficient growth in insect cells. groups of four monkeys received one dose each of test ... | 2012 | 22890035 |
| statins reduce dengue virus production via decreased virion assembly. | most of the effects of statins can be explained by pleiotropic effects independent of their lowering of serum cholesterol; in some cases, these effects have been shown to be a result of the role of statins in the prenylation of cellular proteins, some of which are involved in the life cycle of animal viruses. this study evaluated the potential antiviral activity of lovastatin (lov) against dengue virus (denv) infection of epithelial and endothelial cells (vero cells, epithelial cells derived fro ... | 2011 | 21293097 |
| viremia and antibody response in green monkeys (chlorocebus aethiops sabaeus) infected with dengue virus type 2: a potential model for vaccine testing. | the increasingly limited availability and high cost of the hitherto most commonly used monkey species in dengue vaccine research has augmented the importance of identifying alternative suitable models for these studies. in this study we examined the capacity of green monkeys (chlorocebus aethiops sabaeus) to develop dengue viremia, and thus provide a potential model for dengue vaccine testing. monkeys were inoculated with two different doses of dengue virus type 2. all animals in both groups bec ... | 2009 | 19714858 |
| stimulation of dengue virus replication in cultured aedes albopictus (c6/36) mosquito cells by the antifungal imidazoles ketoconazole and miconazole. | replication of dengue type 3 virus in aedes albopictus c6/36 cells was enhanced more than 50-fold by addition of the antifungal imidazole derivative ketoconazole within the first 4 h of infection. the stimulatory effect was reflected in the yield of infectious virus and in levels of viral rna and protein synthesis. enhanced yields were observed also for other flaviviruses, including dengue type 2 virus and murray valley encephalitis virus. increased yields of dengue type 3 virus were not observe ... | 2000 | 10725192 |
| in vitro analysis of synthetic peptides in blocking the entry of dengue virus. | dengue fever is the most prevalent arthropod-borne viral disease, and no specific therapeutic or promising antiviral drug is available for its treatment. peptide inhibitors are less toxic than synthetic compounds and have found proven effective against viral infections. here, three peptides that mimic part of the e protein of the dengue virus (denv) were synthesized and evaluated for their inhibitory activity against four serotypes of denv in african green monkey kidney (vero) and rhesus macaque ... | 2019 | 30508603 |