| the role of phospholipase in host cell penetration by toxoplasma gondii. | phospholipase a2 (pla2) plays an important pathogenic role in infections caused by several microorganisms and has been implicated in host cell invasion. the mechanism of host cell penetration by the intracellular protozoan toxoplasma gondii involves several steps; we have investigated the role of pla2 in cellular invasion by the tachyzoite stage of this parasite. we assayed t. gondii invasion of human fibroblast monolayers by measurement of the selective incorporation of 3h-uracil into growing i ... | 1989 | 2919724 |
| ohanin, a novel protein from king cobra venom, induces hypolocomotion and hyperalgesia in mice. | we have identified, purified, and determined the complete amino acid sequence of a novel protein, ohanin from ophiophagus hannah (king cobra) venom. it is a small protein containing 107 amino acid residues with a molecular mass of 11951.47 +/- 0.67 da as assessed by electrospray ionization-mass spectrometry. it does not show similarity to any known families of snake venom proteins and hence is the first member of a new family of snake venom proteins. it shows similarity to pry and spry domain pr ... | 2005 | 15668253 |
| antibacterial action of a heat-stable form of l-amino acid oxidase isolated from king cobra (ophiophagus hannah) venom. | the major l-amino acid oxidase (laao, ec 1.4.3.2) of king cobra (ophiophagus hannah) venom is known to be an unusual form of snake venom laao as it possesses unique structural features and unusual thermal stability. the antibacterial effects of king cobra venom laao were tested against several strains of clinical isolates including staphylococcus aureus, staphylococcus epidermidis, pseudomonas aeruginosa, klebsiella pneumoniae, and escherichia coli using broth microdilution assay. for comparison ... | 2010 | 21059402 |
| efficacy of oh-cath30 and its analogs against drug-resistant bacteria in vitro and in mouse models. | antimicrobial peptides (amps) have been considered alternatives to conventional antibiotics for drug-resistant bacterial infections. however, their comparatively high toxicity toward eukaryotic cells and poor efficacy in vivo hamper their clinical application. oh-cath30, a novel cathelicidin peptide deduced from the king cobra, possesses potent antibacterial activity in vitro. the objective of this study is to evaluate the efficacy of oh-cath30 and its analog oh-cm6 against drug-resistant bacter ... | 2012 | 22491685 |
| venom peptides cathelicidin and lycotoxin cause strong inhibition of escherichia coli atp synthase. | venom peptides are known to have strong antimicrobial activity and anticancer properties. king cobra cathelicidin or oh-cath (kf-34), banded krait cathelicidin (bf-30), wolf spider lycotoxin i (il-25), and wolf spider lycotoxin ii (ke-27) venom peptides were found to strongly inhibit escherichia coli membrane bound f1fo atp synthase. the potent inhibition of wild-type e. coli in comparison to the partial inhibition of null e. coli by kf-34, bf-30, il-25, or ke-27 clearly links the bactericidal p ... | 2016 | 26930579 |
| isolation, expression and characterization of a novel dual serine protease inhibitor, oh-tci, from king cobra venom. | snake venom kunitz/bpti members are good tools for understanding of structure-functional relationship between serine proteases and their inhibitors. a novel dual kunitz/bpti serine proteinase inhibitor named oh-tci (trypsin- and chymotrypsin-dual inhibitor from ophiophagus hannah) was isolated from king cobra venom by three chromatographic steps of gel filtration, trypsin affinity and reverse phase hplc. oh-tci is composed of 58 amino acid residues with a molecular mass of 6339da. successful exp ... | 2008 | 18582511 |