| significane of intravascular coagulation in canine endotoxin shock. | the contribution of disseminated fibrin clot formation to the pathogenesis of canine endotoxin shock was explored in control dogs and in those defibrinated with a purified fraction of malayan pit viper venom. the hemodynamic and humoral responses after the administration of an intravenous challenge dose of escherichia coli endotoxin were comparable as was mortality. it is concluded that, although the role of the coagulation sequence in canine endotoxin shock is unclear, it does not appear to be ... | 1975 | 1091556 |
| modulation of rgd sequence motifs regulates disintegrin recognition of alphaiib beta3 and alpha5 beta1 integrin complexes. replacement of elegantin alanine-50 with proline, n-terminal to the rgd sequence, diminishes recognition of the alpha5 beta1 complex with restoration induced by mn2+ cation. | several recent studies have demonstrated that the amino acid residues flanking the rgd sequence of high-affinity ligands modulate their specificity of interaction with integrin complexes. the present study has addressed the role of the residues flanking the rgd sequence in regulating the recognition by disintegrin of the alphaiib beta3 and alpha5beta1 complexes by construction of a panel of recombinant molecules of elegantin (the platelet aggregation inhibitor from the venom of trimerasurus eleg ... | 1998 | 9761721 |
| expression in pichia pastoris and characterization by circular dichroism and nmr of rhodostomin. | rhodostomin (rho) is a snake venom protein isolated from calloselasma rhodostoma. rho is a disintegrin that inhibits platelet aggregation by blocking the binding of fibrinogen to the integrin alpha(iib)beta3 of platelets. rho produced in escherichia coli inhibited platelet aggregation with a k(i) value of 263 nm. although functional, rho produced in e. coli is misfolded based on our 2d and 3d nmr studies. in order to correct the folding problem, rho was expressed in pichia pastoris. the recombin ... | 2001 | 11340665 |