| discovery of a novel coronavirus, china rattus coronavirus hku24, from norway rats supports the murine origin of betacoronavirus 1 and has implications for the ancestor of betacoronavirus lineage a. | we discovered a novel betacoronavirus lineage a coronavirus, china rattus coronavirus (chrcov) hku24, from norway rats in china. chrcov hku24 occupied a deep branch at the root of members of betacoronavirus 1, being distinct from murine coronavirus and human coronavirus hku1. its unique putative cleavage sites between nonstructural proteins 1 and 2 and in the spike (s) protein and low sequence identities to other lineage a betacoronaviruses (βcovs) in conserved replicase domains support chrcov h ... | 2015 | 25552712 |
| isolation and characterization of a novel betacoronavirus subgroup a coronavirus, rabbit coronavirus hku14, from domestic rabbits. | we describe the isolation and characterization of a novel betacoronavirus subgroup a coronavirus, rabbit coronavirus hku14 (rbcov hku14), from domestic rabbits. the virus was detected in 11 (8.1%) of 136 rabbit fecal samples by reverse transcriptase pcr (rt-pcr), with a viral load of up to 10(8) copies/ml. rbcov hku14 was able to replicate in hrt-18g and rk13 cells with cytopathic effects. northern blotting confirmed the production of subgenomic mrnas coding for the he, s, ns5a, e, m, and n prot ... | 2012 | 22398294 |