detection of igg and igm in sera from canines with blastomycosis using eight blastomyces dermatitidis yeast phase lysate antigens. | the objective of this study was to compare the efficacy of eight blastomyces dermatitidis yeast phase lysate antigens (t-58: dog, tennessee; t-27: polar bear, tennessee; erc-2: dog, wisconsin; b5894: human, minnesota; soil: soil, canada; b5896: human, minnesota; 48089: human, zaire; 48938: bat, india) in the detection of the immunoglobulins igg and igm in serum specimens from canines with blastomycosis. an indirect enzyme-linked immunosorbent assay (elisa, peroxidase system) was used to analyze ... | 2006 | 16830189 |
comparative serological evaluation of 10 blastomyces dermatitidis yeast phase lysate antigens from different sources. | yeast phase lysate antigens from 10 isolates of blastomyces dermatitidis (dog, erc-2 and t-58; t-27, polar bear; woodpile, er-3; bat lung, 48938; human, b5929, b5895, b5896, b5931, and capp) from different geographical regions, in addition to a histoplasma capsulatum (g217b) lysate preparation were compared with respect to their reactivity against serum specimens from dogs, rabbits and humans positive for blastomycosis using an indirect enzyme-linked immunosorbent assay. in addition, the lysate ... | 2004 | 15078431 |
blastomyces dermatitidis lysate antigens: antibody detection in serial serum specimens from dogs with blastomycosis. | yeast phase lysate antigens prepared from different isolates of blastomyces dermatitidis (t-58, dog-tennessee; t-27, polar bear-tennessee; erc-2, dog-wisconsin; er-3, woodpile-wisconsin) were compared with respect to the detection of antibodies (indirect enzyme-linked immunosorbent assay-elisa, peroxidase system) in 126 serial serum specimens (pre-treatment, 30 and 60 days post-treatment with itraconazole) from 42 dogs with diagnosed blastomycosis. mean absorbance values observed with the four l ... | 2003 | 14682453 |
comparison of seven blastomyces dermatitidis antigens for the detection of antibodies in humans with occupationally acquired blastomycosis. | yeast phase lysate antigens, prepared from seven isolates of blastomyces dermatitidis (dogs, t-58, m-98; human, le; soil, s; sea lion, sl; polar bear, pb; cat, c) were assayed by enzyme-linked immunosorbent assay (elisa) to detect antibodies in human sera. the sera were from individuals potentially exposed to b. dermatitidis while working on a prairie dog relocation project in colorado. all antigens exhibited greatest reactivity with three specimens in two separate assays. absorbance values rang ... | 2002 | 12572716 |