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detection of igg and igm in sera from canines with blastomycosis using eight blastomyces dermatitidis yeast phase lysate antigens.the objective of this study was to compare the efficacy of eight blastomyces dermatitidis yeast phase lysate antigens (t-58: dog, tennessee; t-27: polar bear, tennessee; erc-2: dog, wisconsin; b5894: human, minnesota; soil: soil, canada; b5896: human, minnesota; 48089: human, zaire; 48938: bat, india) in the detection of the immunoglobulins igg and igm in serum specimens from canines with blastomycosis. an indirect enzyme-linked immunosorbent assay (elisa, peroxidase system) was used to analyze ...200616830189
comparative serological evaluation of 10 blastomyces dermatitidis yeast phase lysate antigens from different sources.yeast phase lysate antigens from 10 isolates of blastomyces dermatitidis (dog, erc-2 and t-58; t-27, polar bear; woodpile, er-3; bat lung, 48938; human, b5929, b5895, b5896, b5931, and capp) from different geographical regions, in addition to a histoplasma capsulatum (g217b) lysate preparation were compared with respect to their reactivity against serum specimens from dogs, rabbits and humans positive for blastomycosis using an indirect enzyme-linked immunosorbent assay. in addition, the lysate ...200415078431
blastomyces dermatitidis lysate antigens: antibody detection in serial serum specimens from dogs with blastomycosis.yeast phase lysate antigens prepared from different isolates of blastomyces dermatitidis (t-58, dog-tennessee; t-27, polar bear-tennessee; erc-2, dog-wisconsin; er-3, woodpile-wisconsin) were compared with respect to the detection of antibodies (indirect enzyme-linked immunosorbent assay-elisa, peroxidase system) in 126 serial serum specimens (pre-treatment, 30 and 60 days post-treatment with itraconazole) from 42 dogs with diagnosed blastomycosis. mean absorbance values observed with the four l ...200314682453
comparison of seven blastomyces dermatitidis antigens for the detection of antibodies in humans with occupationally acquired blastomycosis.yeast phase lysate antigens, prepared from seven isolates of blastomyces dermatitidis (dogs, t-58, m-98; human, le; soil, s; sea lion, sl; polar bear, pb; cat, c) were assayed by enzyme-linked immunosorbent assay (elisa) to detect antibodies in human sera. the sera were from individuals potentially exposed to b. dermatitidis while working on a prairie dog relocation project in colorado. all antigens exhibited greatest reactivity with three specimens in two separate assays. absorbance values rang ...200212572716
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