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estimation of ursodeoxycholic acid in human and bear biles using clostridium absonum 7 beta-hydroxysteroid dehydrogenase.ursodeoxycholic acid was estimated in bile samples from humans and wild north american black bears using 7 beta-hydroxysteroid dehydrogenase purified from clostridium absonum by procion red affinity chromatography. the percentage ursodeoxycholic acid was calculated by two methods: (a) 7 beta-hydroxyl groups were quantified using 7 beta-hydroxysteroid dehydrogenase and 3 alpha-hydroxyl groups (total bile acids) were quantified using 3 alpha-hydroxysteroid dehydrogenase. the percentage ursodeoxych ...19836581749
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