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toxoplasma gondii, neospora caninum, sarcocystis neurona, and sarcocystis canis-like infections in marine mammals.toxoplasma gondii, neospora caninum, sarcocystis neurona, and s. canis are related protozoans that can cause mortality in many species of domestic and wild animals. recently, t. gondii and s. neurona were recognized to cause encephalitis in marine mammals. as yet, there is no report of natural exposure of n. caninum in marine mammals. in the present study, antibodies to t. gondii and n. caninum were assayed in sera of several species of marine mammals. for t. gondii, sera were diluted 1:25, 1:50 ...200314580799
spatio-temporal variations and age effect on toxoplasma gondii seroprevalence in seals from the canadian arctic.summarytoxoplasmosis is a significant public health threat for inuit in the canadian arctic. this study aimed to investigate arctic seals as a possible food-borne source of infection. blood samples collected from 828 seals in 7 canadian arctic communities from 1999 to 2006 were tested for toxoplasma gondii antibodies using a direct agglutination test. polymerase chain reaction (pcr) was used to detect t. gondii dna in tissues of a subsample of seals. associations between seal age, sex, species, ...201121813043
serosurvey of toxoplasma gondii in north atlantic marine mammals by the use of agglutination test employing whole tachyzoites and dithiothreitol.serum samples from north atlantic populations of harp seal, phoca groenlandica (n = 316), ringed seal, phoca hispida (n = 48), hooded seal, cystophora cristata (n = 78), and minke whale, balaenoptera acutorostrata (n = 202), were tested for toxoplasma gondii-specific igg. the modified agglutination test (mat) was slightly modified to be more user-friendly by replacing the 0.2 m 2-mercaptoethanol with 10 mm dithiothreitol. no positive samples were found at 1:40 dilution.19989611681
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