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evaluation of campylobacter jejuni colonization of the domestic ferret intestine as a model of proliferative colitis.forty 3- to 17-week old domestic ferrets, including 2 gnotobiotes, were inoculated orally and/or rectally with 10(6) to 10(9) colony-forming units of 1 or more of 4 strains of campylobacter jejuni, 3 of mink and 1 of human origin. feeding or gavage of any of the 4 strains, in milk or broth, with or without preinoculation sodium bicarbonate treatment to neutralize stomach acid, induced colonization in 38/40 ferrets; diarrhea lasted 2 to 4 days in conventional kits, 6 days in gnotobiotes. bacterem ...19911883085
reproductive failure in mink and ferrets after intravenous or oral inoculation of campylobacter jejuni.four pregnant mink and seven pregnant ferrets, including five with previous exposure and specific antibody, were injected intravenously with 10(8)-10(10) colony-forming units of campylobacter jejuni. all 11 pregnancies failed 1-16 days after infection, with results ranging from fetal resorption to expulsion of dead or premature living kits. in every case, uterine contents (placenta, uterine fluid and/or kits) were culture-positive for c. jejuni. three pregnant mink and nine pregnant ferrets, inc ...19902249178
a domestic ferret model of immunity to campylobacter jejuni-induced enteric disease.oral or intravenous inoculation of previously unexposed juvenile and adult ferrets with campylobacter jejuni uniformly resulted in intestinal colonization lasting 2 to 12 days. disease varied from mild to moderate diarrhea, which resolved in 2 to 3 days. orally infected animals developed agglutinin titers of 8 to 256 within 3 weeks, while those infected intravenously developed titers of 256 to 2,048. ferrets which had recovered from campylobacteriosis all developed high titers of agglutinating a ...19902341180
microbiological aspects of helicobacter pylori (campylobacter pylori).the human gastric pathogen campylobacter pylori has recently been reclassified as helicobacter pylori, and a related spiral bacterium found in the stomach of ferrets has been designated helicobacter mustelae. the general microbiological features of helicobacter pylori are delineated here, with details of phenotypic differences between helicobacter pylori and helicobacter mustelae; comparisons are made with wolinella succinogenes and campylobacter jejuni. the helicobacter organisms possess an ext ...19902406141
diversity of serotypes of campylobacter jejuni and campylobacter coli isolated in laboratory animals.one hundred nineteen isolates of campylobacter jejuni and campylobacter coli from nine laboratory animal species were serotyped using antisera to 20 penner serotypes commonly isolated from cases of human enteric infections. although c. jejuni and c. coli were isolated from laboratory animals with diarrhea, the majority were cultured from asymptomatic animals (81%). seven of twenty-two isolates from animals with diarrhea were serotype 4 (32%) and three were serotype 1 (14%). sixty-one of the 119 ...19892724920
campylobacter jejuni infection in the ferret: an animal model of human campylobacteriosis.campylobacter infection in weanling ferrets (mustela putorius furo) was studied as an animal model for enteric campylobacteriosis in persons. the screening of fecal cultures on selective campylobacter media showed that campylobacter jejuni/coli was not present in the normal enteric flora. intragastric feeding of a mixture of cat feed and 2.5 x 10(8) c jejuni isolated from ferrets with naturally occurring proliferative colitis was accomplished. all ferrets (n = 8) became infected on 3 days after ...19873826848
ferret as a potential reservoir for human campylobacteriosis.a survey was conducted to determine the frequency of campylobacter fetus subsp jejuni in feces of ferrets purchased for use in biomedical research. over a 12-month period, 168 ferrets from 2 commercial breeders were examined by bacteriologic cultural procedures for campylobacter spp; 61% were culturally positive for c fetus subsp jejuni. in a therapeutic trial with 16 ferrets shedding c fetus subsp jejuni in feces, erythromycin given orally failed to eliminate intestinal carriage of the organism ...19836870007
proliferative colitis in ferrets.during a 4-month period, 31 of 156 ferrets (mustela putorius) in a biomedical research program developed protracted diarrhea. clinical signs were green mucohemorrhagic fecal material, partially prolapsed rectum, anorexia, body weight loss, and dehydration. nine of the affected animals were necropsied. on gross examination, the descending colon was grossly thick and histologically characterized by marked proliferation of the mucosa, relatively few goblet cells, mixed inflammatory cell infiltrate, ...19827091850
an environmentally regulated pilus-like appendage involved in campylobacter pathogenesis.examination of strains of campylobacter jejuni, campylobacter coli, and campylobacter fetus by electron microscopy revealed that they produced peritrichous pilus-like appendages when the bacteria were grown in the presence of bile salts. various bile-salt supplements were used and it was found that deoxycholate and chenodeoxycholic acid caused a significant enhancement of pilus production and resulted in a highly aggregative phenotype. morphologically, the pili were between 4 and 7 nm in width a ...19968793884
chey-mediated modulation of campylobacter jejuni virulence.four motile, non-adherent and non-invasive mutants of campylobacter jejuni 81-176 generated by a site-specific insertional mutagenesis scheme were characterized at the molecular level and all contained a duplication of the same region of the chromosome. when this region was cloned from wild-type 81-176 and transferred into 81-176 on a shuttle plasmid, the same non-invasive phenotype as the original mutants was observed, suggesting that the region contained a repressor of adherence and invasion. ...19979076738
involvement of a plasmid in virulence of campylobacter jejuni 81-176.campylobacter jejuni strain 81-176 contains two, previously undescribed plasmids, each of which is approximately 35 kb in size. although one of the plasmids, termed ptet, carries a teto gene, conjugative transfer of tetracycline resistance to another strain of c. jejuni could not be demonstrated. partial sequence analysis of the second plasmid, pvir, revealed the presence of four open reading frames which encode proteins with significant sequence similarity to helicobacter pylori proteins, inclu ...200010899834
a phase-variable capsule is involved in virulence of campylobacter jejuni 81-176.campylobacter jejuni strain 81-176 (hs36, 23) synthesizes two distinct glycan structures, as visualized by immunoblotting of proteinase k-digested whole-cell preparations. a site-specific insertional mutant in the kpsm gene results in loss of expression of a high-molecular-weight (hmw) glycan (apparent mr 26 kda to > 85 kda) and increased resolution of a second ladder-like glycan (apparent mr 26-50 kda). the kpsm mutant of 81-176 is no longer typeable in either hs23 or hs36 antisera, indicating ...200111359581
prevention of disease in ferrets fed an inactivated whole cell campylobacter jejuni vaccine.ferrets were used to demonstrate the potential of a killed whole cell vaccine prepared from campylobacter jejuni to protect against disease. c. jejuni strain 81-176 was grown in bhi broth, formalin-fixed, and resuspended in pbs to a concentration of 10(10) cells per ml. this vaccine (cwc) or live organisms were delivered orally with a nasogastric tube into anesthetized animals treated to reduce gastric acidity and intestinal motility. when 5x10(10) cfu of the vaccine strain (lior serotype 5) or ...200516005742
a sigma28-regulated nonflagella gene contributes to virulence of campylobacter jejuni 81-176.a campylobacter jejuni 81-176 mutant in cj0977 was fully motile but reduced >3 logs compared to the parent in invasion of intestinal epithelial cells in vitro. the mutant was also attenuated in a ferret diarrheal disease model. expression of cj0977 protein was dependent on a minimal flagella structure.200616369037
changes in flagellin glycosylation affect campylobacter autoagglutination and virulence.analysis of the complete flagellin glycosylation locus of campylobacter jejuni strain 81-176 revealed a less complex genomic organization than the corresponding region in the genome strain, c. jejuni nctc 11168. twenty-four of the 45 genes found between cj1293 and cj1337 in nctc 11168 are missing in 81-176. mutation of six new genes, in addition to three previously reported, resulted in a non-motile phenotype, consistent with a role in synthesis of pseudaminic acid (pseac) or transfer of pseac t ...200616573682
genome sequence of a clinical isolate of campylobacter jejuni from thailand.campylobacter jejuni cg8486, which belongs to the hs4 complex, was isolated from a patient with inflammatory diarrhea in thailand. this strain caused a diarrheal disease in ferrets comparable to that caused by c. jejuni strain 81-176, but it was much less invasive for epithelial cells in vitro than 81-176. complete genome sequencing of cg8486 revealed a 1.65-mb genome that was very similar to the other two published genomes of clinical isolates of c. jejuni, the genomes of 81-176 and nctc 11168, ...200717438034
immune response to and histopathology of campylobacter jejuni infection in ferrets (mustela putorius furo).campylobacter jejuni is 1 of the most common enteric bacterial pathogens worldwide. the mechanisms of pathogenesis remain obscure, in part because of limitations of small animal models. young ferrets develop diarrhea when fed c. jejuni, but their pathology and the immune response after infection have not been examined in detail. in the present study, we examined the pathogenesis of c. jejuni cg8421 and associated immune responses in ferrets. after oral infection with c. jejuni cg8421, 86.7% of t ...200919712577
comparative genomics and proteomics of helicobacter mustelae, an ulcerogenic and carcinogenic gastric pathogen.helicobacter mustelae causes gastritis, ulcers and gastric cancer in ferrets and other mustelids. h. mustelae remains the only helicobacter other than h. pylori that causes gastric ulceration and cancer in its natural host. to improve understanding of h. mustelae pathogenesis, and the ulcerogenic and carcinogenic potential of helicobacters in general, we sequenced the h. mustelae genome, and identified 425 expressed proteins in the envelope and cytosolic proteome.201020219135
campylobacter polysaccharide capsules: virulence and vaccines.campylobacter jejuni remains a major cause of bacterial diarrhea worldwide and is associated with numerous sequelae, including guillain barré syndrome, inflammatory bowel disease, reactive arthritis, and irritable bowel syndrome. c. jejuni is unusual for an intestinal pathogen in its ability to coat its surface with a polysaccharide capsule (cps). these capsular polysaccharides vary in sugar composition and linkage, especially those involving heptoses of unusual configuration and o-methyl phosph ...201222919599
the human milk oligosaccharide 2'-fucosyllactose quenches campylobacter jejuni-induced inflammation in human epithelial cells hep-2 and ht-29 and in mouse intestinal mucosa.campylobacter jejuni causes diarrhea worldwide; young children are most susceptible. binding of virulent c. jejuni to the intestinal mucosa is inhibited ex vivo by α1,2-fucosylated carbohydrate moieties, including human milk oligosaccharides (hmoss).201627629573
using galleria mellonella as an infection model for campylobacter jejuni pathogenesis.nonmammalian model systems of infection have been employed recently to study bacterial virulence. for instance, galleria mellonella (the greater wax moth) has been shown to be susceptible to infection by many bacterial pathogens including the enteric pathogen campylobacter jejuni. in contrast to the traditional animal models for c. jejuni such as the chick colonization model and ferret diarrheal model, the galleria mellonella infection model has the advantages of lower cost, ease of use and no a ...201727885606
transmigration route of campylobacter jejuni across polarized intestinal epithelial cells: paracellular, transcellular or both?intact intercellular junctions and cellular matrix contacts are crucial structural components for the formation and maintenance of epithelial barrier functions in humans to control the commensal flora and protect against intruding microbes. campylobacter jejuni is one of the most important zoonotic pathogens causing food-borne gastroenteritis and potentially more severe diseases such as reactive arthritis or guillain-barré syndrome. crossing the intestinal epithelial barrier and host cell invasi ...201324079544
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