| high prevalence of a novel porcine bocavirus in weanling piglets with respiratory tract symptoms in china. | this study presents the first evidence of infection by a novel porcine bocavirus (pbov) in chinese swine herds. the pcr detection results showed that pbov was significantly more prevalent in weanling piglets (69.7%, 69/99) with respiratory tract symptoms than that in other samples (0-13.6%) (p < 0.01). sequence analysis showed that the partial vp1/2 genes were highly conserved (99-100% identity), and only five frequent nucleotide mutation positions existed in chinese pbov strains. these data ind ... | 2010 | 20495986 |
| identification and nearly full-length genome characterization of novel porcine bocaviruses. | the genus bocavirus includes bovine parvovirus (bpv), minute virus of canines (mvc), and a group of human bocaviruses (hbov1-4). using sequence-independent single primer amplification (sispa), a novel bocavirus group was discovered with high prevalence (12.59%) in piglet stool samples. two nearly full-length genome sequences were obtained, which were approximately 5,100 nucleotides in length. multiple alignments revealed that they share 28.7-56.8% dna sequence identity with other members of parv ... | 2010 | 21049037 |
| complete coding sequences and phylogenetic analysis of porcine bocavirus. | here we report, for the first time, the nearly full-length genome sequence of porcine bocavirus (pbov), a recently discovered parvovirus from pigs. phylogenetic trees based on this genome sequence showed that pbov belongs to the branch containing the genus bocavirus, which comprises canine minute virus (cnmv), bovine parvovirus, gorilla bocavirus and human bocavirus (hbov), and was most closely related to the group containing cnmv. pbov was predicted to contain three potential orfs encoding the ... | 2011 | 21228124 |
| recombination analysis based on the complete genome of bocavirus. | abstract: bocavirus include bovine parvovirus, minute virus of canine, porcine bocavirus, gorilla bocavirus, and human bocaviruses 1-4 (hbovs). although recent reports showed that recombination happened in bocavirus, no systematical study investigated the recombination of bocavirus. the present study performed the phylogenetic and recombination analysis of bocavirus over the complete genomes available in genbank. results confirmed that recombination existed among bocavirus, including the likely ... | 2011 | 21507266 |
| genomic characterization and high prevalence of bocaviruses in swine. | using random pcr amplification followed by plasmid subcloning and dna sequencing, we detected bocavirus related sequences in 9 out of 17 porcine stool samples. using primer walking, we sequenced the nearly complete genomes of two highly divergent bocaviruses we provisionally named porcine bocavirus 1 isolate h18 (pbov1-h18) and porcine bocavirus 2 isolate a6 (pbov2-a6) which differed by 51.8% in their ns1 protein. phylogenetic analysis indicated that pbov1-h18 was very closely related to a ∼2 kb ... | 2011 | 21525999 |
| isolation in cell cultures and initial characterisation of two novel bocavirus species from swine in northern ireland. | we report the isolation in cell cultures of two novel bocavirus species in pigs from farms in northern ireland with clinical postweaning multisystemic wasting syndrome (pmws). we have designated the isolates as porcine bocavirus-3 (pbov3) and porcine bocavirus-4 (pbov4). to date 5082 and 4125bps of pbov3 and pbov4 have been sequenced, respectively. pbov3 and pbov4 show nucleotide homology to other known bocaviruses in swine and other organisms. open reading frame (orf) analysis has shown that th ... | 2011 | 21605951 |
| co-existence of multiple strains of two novel porcine bocaviruses in the same pig, a previously undescribed phenomenon in parvoviridae and evidence for inter- and intra-host genetic diversity and recombination. | despite the recent discovery of novel bocaviruses from porcine samples, their genetic evolution and diversity are poorly understood. we report the identification and complete genome characterization of two novel parvoviruses, porcine bocavirus 3 (pbov3) and porcine bocavirus 4 (pbov4), from various porcine tissues/samples, which displayed marked intra- and inter-host genetic diversity, with recombination events. bocaviruses were detected by pcr among 16.5 % (55/333) of porcine samples (including ... | 2011 | 21632566 |
| genetic detection and analysis of porcine bocavirus type 1 (pobov1) in european wild boar (sus scrofa). | novel porcine parvoviruses showing the genetic characteristics of bocaviruses have recently been identified. the first such porcine bocavirus (pobov1), described as boca-like virus (pbo-likev), was discovered in pmws affected pigs in sweden. later, several other bocaviruses with divergent genomes were reported under various names in domestic pigs. this is the first report of the presence of bocaviruses in european wild boars. 842 wild boar samples originating from the western region of romania ( ... | 2011 | 21822671 |
| Complete Genome Sequence of a Novel Species of Porcine Bocavirus, PBoV5. | Porcine bocavirus 5 is a novel porcine bocavirus species found in a pig with clinical diarrhea from a farm in China. Here, we report the complete genome sequence of strain PBoV5/JS677, which will help toward understanding the molecular and evolutionary characteristics of the porcine bocavirus. | 2012 | 22205722 |
| emerging and re-emerging swine viruses. | in the past two decades or so, a number of viruses have emerged in the global swine population. some, such as porcine reproductive and respiratory syndrome virus (prrsv) and porcine circovirus type 2 (pcv2), cause economically important diseases in pigs, whereas others such as porcine torque teno virus (ttv), now known as torque teno sus virus (ttsuv), porcine bocavirus (pbov) and related novel parvoviruses, porcine kobuvirus, porcine toroviruses (ptov) and porcine lymphotropic herpesviruses (pl ... | 2012 | 22225855 |
| porcine bocavirus infection associated with encephalomyelitis in a pig, germany(1). | | 0 | 27315461 |
| a new nanopcr molecular assay for detection of porcine bocavirus. | nanoparticle-assisted polymerase chain reaction (nanopcr) is a novel method for the rapid amplification of dna and has been used for the detection of virus. for detection of porcine bocavirus (pbov), a sensitive and specific nanopcr assay was developed with a pair of primers that were designed based on ns1 gene sequences available in genbank. under the optimized conditions of the pbov nanopcr assay, the nanopcr assay was 100-fold more sensitive than a conventional pcr assay. the lower detection ... | 2014 | 24642242 |
| detection and genetic analysis of porcine bocavirus in different swine herds in north central china. | porcine bocavirus (pbov) has been reported to be associated with postweaning multisystemic wasting syndrome and pneumonia in pigs. in this study, a survey was conducted to evaluate the prevalence of pbov in slaughter pigs, sick pigs, asymptomatic pigs and classical swine fever virus (csfv) eradication plan herds in five provinces of china (henan, liaoning, shandong, hebei and tianjin) by means of pcr targeting ns1 gene of pbov. among the total of 403 tissue samples, 11.41% were positive for pbov ... | 2014 | 24701194 |
| development of a loop-mediated isothermal amplification assay for the detection of porcine hokovirus. | hokoviruses have recently been detected as pathogens belonging to the family parvoviridae, which comprises porcine hokovirus (phov) and bovine hokovirus (bhov). in this study, we developed a loop-mediated isothermal amplification (lamp) assay for the rapid, specific and sensitive detection of phov. a set of four primers specific for six regions within the phov vp1/2 genes was designed using online software. the reaction temperature and time were optimized at 65°c and 60 min, respectively. lamp p ... | 2013 | 23850717 |
| [metagenomics-based detection of swine viruses]. | extreme varieties of viruses exist in the environment and animals, some of which are unknown. however, many unknown viruses are barely detected by means of conventional virus isolation and pcr assay. | 2013 | 23627112 |
| detection and characterisation of novel bocavirus (genus bocaparvovirus) and gastroenteritis viruses from asymptomatic pigs in ireland. | livestock animals have been the assumed source of several human epidemics in recent years, for example, influenza h1n1, rotavirus g8/g9, and mers-cov. surveillance of novel viruses in animals is essential to evaluate the risk to human and animal health and to determine any economic impact, for example, failure to thrive. there is a paucity of data regarding detection and characterisation of gastroenteritis viruses, particularly novel viruses, in porcines in ireland. recently, a number of small n ... | 2015 | 26065833 |
| first identification of porcine parvovirus 6 in north america by viral metagenomic sequencing of serum from pigs infected with porcine reproductive and respiratory syndrome virus. | currently, eight species in four genera of parvovirus have been described that infect swine. these include ungulate protoparvovirus 1 (classical porcine parvovirus, ppv), ungulate tetraparvovirus 2 (ppv3), ungulate tetraparvovirus 3 (which includes ppv2, porcine hokovirus, porcine partetravirus and porcine parv4), ungulate copiparvovirus 2 (which includes ppv4 and ppv5), ungulate bocaparvovirus 2 (which includes porcine bocavirus 1, 2 and 6), ungulate bocaparvovirus 3 (porcine bocavirus 5), ungu ... | 2015 | 26475593 |
| virus-like particles of porcine bocavirus generated by recombinant baculoviruses can be applied to sero-epidemic studies. | porcine bocaviruses (pbovs), new members of the bocavirus genus, have been identified in swine worldwide. however, the antigenicity and epidemiology of pbovs are still unclear. here we used a recombinant baculovirus expression system to express the main capsid protein vp2 of japan strain jy31b in insect tn5 cells, and successfully produced the virus-like particles of pbov (pbov-lps). the diameter and densities of the pbov-lps were estimated to be 30nm and 1.300g/cm(3), respectively, which were s ... | 2016 | 26959654 |
| viral metagenomics analysis demonstrates the diversity of viral flora in piglet diarrhoeic faeces in china. | to investigate the diversity of viral flora, we used metagenomics to study the viral communities in a pooled faecal sample of 27 diarrhoeic piglets from intensive commercial farms in china. the 15 distinct mammalian viruses identified in the pooled diarrhoeic sample were, in order of abundance of nucleic acid sequence, porcine epidemic diarrhea virus (pedv), sapovirus, porcine bocavirus-4 (pbov-4), sapelovirus, torovirus, coronavirus, pbov-2, stool-associated single-stranded dna virus (poscv), a ... | 2014 | 24718833 |
| detection, prevalence and analysis of emerging porcine parvovirus infections. | a number of newly identified porcine parvoviruses had been described during the last decade, but the presence and prevalence of these viruses are unknown in hungary and only partly known for europe. the present study was conducted to detect and measure the prevalence of these viruses, namely porcine parvovirus (ppv) 2, ppv3, ppv4, porcine bocavirus (pbov) 1, pbov2, pbo-likev and the 6v and 7v parvoviruses. the prevalence of ppv1 and porcine circovirus type 2 (pcv2) was also investigated. faecal ... | 2012 | 22383055 |
| occurrence and investigation of enteric viral infections in pigs with diarrhea in china. | between february 2011 and february 2012, 985 and 324 samples were collected from diarrheal and healthy pigs, respectively, to detect porcine epidemic diarrhea virus (pedv), porcine kobuvirus (pkov), porcine bocavirus (pbov), porcine group a rotavirus (garv), and transmissible gastroenteritis virus (tgev). pedv and pkov clearly predominated in diarrheal pigs. compared to healthy pigs, a substantial prevalence of mixed infections was observed in diarrheal pigs (72.3 %) (p < 0.001). all of the coin ... | 2013 | 23494226 |
| porcine bocavirus np1 protein suppresses type i ifn production by interfering with irf3 dna-binding activity. | type i interferon (ifn) and the ifn-induced cellular antiviral responses are the primary defense mechanisms against viral infection; however, viruses always evolve various mechanisms to antagonize this host's ifn responses. porcine bocavirus (pbov) is a newly identified porcine parvovirus. in this study, we found that the nonstructural protein np1 of pbov inhibits sendai virus-induced ifn-β production and the subsequent expression of ifn-stimulating genes (isgs). ectopic expression of np1 signif ... | 2016 | 27481269 |
| development and validation of a multiplex conventional pcr assay for simultaneous detection and grouping of porcine bocaviruses. | porcine bocavirus (pbov), a newly described porcine parvovirus, has received attention because it can be commonly identified in clinically affected pigs including pigs with post-weaning multisystemic wasting syndrome (pwms) and pigs with diarrhea. in recent years, novel pbovs have been identified and were classified into three genogroups, but the ability to detect and classify these novel pbovs is not comprehensive to date. in this study, a multiplex conventional pcr assay for simultaneous detec ... | 2016 | 27448821 |
| porcine bocavirus np1 negatively regulates interferon signaling pathway by targeting the dna-binding domain of irf9. | to subvert host antiviral immune responses, many viruses have evolved countermeasures to inhibit ifn signaling pathway. porcine bocavirus (pbov), a newly identified porcine parvovirus, has received attention because it shows clinically high co-infection prevalence with other pathogens in post-weaning multisystemic wasting syndrome (pwms) and diarrheic piglets. in this study, we screened the structural and non-structural proteins encoded by pbov and found that the non-structural protein np1 signi ... | 2015 | 26342467 |
| concurrent infections of pseudorabies virus and porcine bocavirus in china detected by duplex nanopcr. | nanoparticle-assisted polymerase chain reaction (nanopcr) is a novel method for the simple, rapid, and specific amplification of dna and has been used to detect viruses. a duplex nanopcr molecular detection system was developed to detect pseudorabies virus (prv) and porcine bocavirus (pbov). primers were selected to target conserved regions within the prv ge gene and the pbov ns1 gene. under optimized nanopcr reaction conditions, two specific fragments of 316 bp (prv) and 996 bp (pbov) were ampl ... | 2015 | 25813598 |
| a viral metagenomic approach on a non-metagenomic experiment: mining next generation sequencing datasets from pig dna identified several porcine parvoviruses for a retrospective evaluation of viral infections. | shot-gun next generation sequencing (ngs) on whole dna extracted from specimens collected from mammals often produces reads that are not mapped (i.e. unmapped reads) on the host reference genome and that are usually discarded as by-products of the experiments. in this study, we mined ion torrent reads obtained by sequencing dna isolated from archived blood samples collected from 100 performance tested italian large white pigs. two reduced representation libraries were prepared from two dna pools ... | 2017 | 28662150 |
| a novel porcine kobuvirus emerged in piglets with severe diarrhoea in china. | since the first report of porcine kobuvirus (pkv) in 2008, it has been confirmed that pkv is widely present in several countries and plays an important role in diarrhoea outbreak in pigs. studies have shown that the biggest difference among pkvs is the presence or absence of a specific 30-amino acid (aa) sequence in the 2b region of the polyprotein gene. based on this unique feature, most pkv sequences could be divided into two groups (group 1 without deletion and group 2 with deletion), but a f ... | 2017 | 28497552 |
| the silent spread of porcine bocavirus in croatian pigs: should we be concerned? | a survey was conducted to evaluate the presence and prevalence of porcine bocavirus (pbov) in croatian domestic pigs by means of pcr targeting the ns1 gene fragment of pbov. this study included testing of faecal samples collected from 10 small commercial farms and 11 small backyard holdings in croatia. the presence of pbov was confirmed by pcr in 24 out of 57 composite faecal samples from small commercial farms and in 12 out of 43 composite faecal samples from small backyard holdings. the pcr pr ... | 2017 | 29256289 |
| a novel porcine bocavirus harbors a variant np gene. | porcine bocavirus is classified within the genus bocaparvovirus, family parvoviridae. unlike other parvoviruses, the members of genus bocaparvovirus (bocaparvoviruses) encode an additional open reading frame (np1). many strains of pbovs have been identified in domestic pigs and recognized as a potential emerging pathogen causing respiratory and gastrointestinal disease. | 2015 | 26217547 |
| molecular detection and genetic analysis of porcine bocavirus in korean domestic swine herds. | several porcine bocaviruses have been detected worldwide, and this report is the first to describe this virus in a korean domestic swine herd. we identified porcine bocavirus in various samples, including serum, tissue, feces and saliva, which revealed that porcine bocavirus predominates in korean domestic swine populations. the results of this study also suggested that porcine bocaviruses primarily infect weaned piglets. phylogenetic analysis of the orf3 gene was performed to determine the gene ... | 2014 | 24352435 |
| genetic characterisation of a porcine bocavirus detected in domestic pigs in uganda. | porcine bocaviruses (pobovs) are small linear ssdna viruses belonging to the genus bocavirus in the family parvoviridae. the genome encodes four proteins-the non-structural protein 1 (ns1), the np1 protein (unknown function) and the two structural proteins vp1 and vp2. in recent years, a number of different highly divergent pobov species have been discovered. pobovs have been shown to be present in pig populations in europe, asia and in the united states of america. in this study, we present the ... | 2013 | 23225112 |
| first molecular detection of porcine bocavirus in malaysia. | several strains of porcine bocaviruses have been reported worldwide since their first detection in sweden in 2009. subsequently, the virus has been reported to be associated with gastrointestinal and respiratory signs in weaner and grower pigs. although malaysia is host to a self-sufficient swine livestock industry, there is no study that describes porcine bocavirus in the country. this report is the first to describe porcine bocavirus (pbov) in malaysian swine herds. pbov was identified in vari ... | 2017 | 29243138 |
| genome sequence of a porcine bocavirus detected in feces of domestic minks in china. | we report here the genome sequence of porcine bocavirus strain pbov-jz08, which was isolated from mink feces in china. sequence analysis implied that pbov-jz08 clustered with three porcine bocaviruses. | 2017 | 29122866 |
| identification of an enterovirus recombinant with a torovirus-like gene insertion during a diarrhea outbreak in fattening pigs. | diarrhea outbreaks in pig farms have raised major concerns in europe and usa, as they can lead to dramatic pig losses. during a suspected outbreak in belgium of porcine epidemic diarrhea virus (pedv), we performed viral metagenomics to assess other potential viral pathogens. although pedv was detected, its low abundance indicated that other viruses were involved in the outbreak. interestingly, a porcine bocavirus and several enteroviruses were most abundant in the sample. we also observed the pr ... | 2017 | 28924489 |
| first complete genome sequences of porcine bocavirus strains from east africa. | here, we report the first complete genome sequences of two strains of porcine bocavirus (joa_011 and joa_015) detected in uganda and kenya, respectively. these data will help in understanding the molecular and evolutionary characteristics of the porcine bocaviruses in this region and the development of appropriate diagnostic and control tools. | 2017 | 28385838 |
| differential contributions of porcine bocavirus np1 protein n- and c-terminal regions to its nuclear localization and immune regulation. | porcine bocavirus (pbov), a newly identified parvovirus in the family parvoviridae, has been reported worldwide in swine with post-weaning multisystemic wasting syndrome, respiratory disease or diarrhoea and in asymptomatic swine. np1 is a protein unique to the genus bocavirus and its function is not fully understood. in this study, we show that the n-terminal region of pbov np1 contains two classical nuclear localization signals (cnlss) and a non-classical nls. the n-terminal region also inhibi ... | 2016 | 26813332 |
| porcine bocavirus: achievements in the past five years. | porcine bocavirus is a recently discovered virus that infects pigs and is classified within the bocavirus genus (family parvoviridae, subfamily parvovirinae). the viral genome constitutes linear single-stranded dna and has three open reading frames that encode four proteins: ns1, np1, vp1, and vp2. there have been more than seven genotypes discovered to date. these genotypes have been classified into three groups based on vp1 sequence. porcine bocavirus is much more prevalent in piglets that are ... | 2014 | 25514206 |
| evolutionary, epidemiological, demographical, and geographical dissection of porcine bocavirus in china and america. | porcine bocavirus was first discovered in swedish pigs with post-weaning multisystemic wasting syndrome (pmws) in 2009. many efforts have been implemented to investigate the porcine bocavirus, but it remains enigmatic. in the current study, we utilized data from both china and the usa. the china-derived data included 403 pig samples collected from five provinces, 122 gene sequences from the genbank database, and 637 old porcine bocavirus (pbov) cases. the usa-derived data comprised 181 pig sampl ... | 2015 | 25289962 |
| complete sequence and phylogenetic analysis of a porcine bocavirus strain swbov ch437. | porcine bocavirus (pbov), a member of genus bocavirus, family parvoviridae, was first identified in 2009 in swedish swine herds suffering from postweaning multisystemic wasting syndrome. up to date, the different species of pbovs have been reported in different countries. especially, the virus isolated in china was complicated. in this study, we detected a novel pbov strain swbov ch437 from clinical samples collected in gansu province, northwest china. the complete genome of swbov ch437 was 5,27 ... | 2014 | 24469465 |
| detection and characterization of porcine bocavirus in the united states. | we screened pigs (n = 203) presenting with respiratory illness or diarrhea for porcine bocavirus (pbov); 88 (43.30 %) were positive by pcr. more positives were seen in diarrhea cases (48.7 %) than in respiratory cases (29.1 %). based on phylogenetic analysis of 540 nucleotides of the ns1 gene, the viruses could be divided into four possible groups. group iv sequences did not match any genbank sequences, while groups i, ii and iii gave matches with pbov3, pbov4 and pbov5, respectively. the wide r ... | 2014 | 24445812 |
| [identification and characterization of porcine bocavirus episomes]. | to verify that the circular forms of bocavirus genome exist in their host, bocavirus episomes were detected in fecal samples of healthy piglets using a semi-nested pcr method. two species of porcine bocaviruses (pbovg2-episome and pbovg3-episome) were identified for the first time. the relevant terminal sequences of the noncoding region (405 and 511 nt, respectively) were also obtained. sequence analyses and secondary structure prediction indicated that the pbovg2-episome was more similar to tha ... | 2012 | 22978168 |
| genome characterization of a novel porcine bocavirus. | using a high-throughput dna sequencing method, one dna sequence (contig01006), suspected to belong to a novel porcine bocavirus (pbov), was found with a high rate of detection (19.6 %) in fecal samples from healthy piglets. moreover, a novel pbov (tentatively named pbov3c) with a nearly complete genome sequence (5235 bp) was identified. pbov3c exhibits typical genome characteristics of bocaviruses and shows the highest genomic sequence identity (78 % to 81 %) to pbov3a/b (pbov3/4-uk) and pbov3d/ ... | 2012 | 22821184 |
| [research advances in porcine bocavirus]. | porcine bocavirus (pbov) was considered as a new member of the genus bocavirus of the subfamily parvovirinae of the family parvoviridae, which was discovered in swedish swine herds with postweaning multisystemic wasting syndrome (pmws) in 2009. at present, as an emerging pathogen, it was paid great attention by researchers at home and abroad. this paper referred to some published literatures and reviewed several aspects of pbov including its finding, classification, genome structure and replicat ... | 2012 | 22519183 |