| detection of a novel porcine boca-like virus in the background of porcine circovirus type 2 induced postweaning multisystemic wasting syndrome. | porcine circovirus type 2 (pcv-2) has been found to be the causative agent of postweaning multisystemic wasting syndrome (pmws). however, pcv-2 is a ubiquitous virus in the swine population and a majority of pigs infected with pcv-2 do not develop the disease. different factors such as age, maintenance, the genetics of pcv-2, other pathogens, etc. have been suggested to contribute to the development of pmws. however, so far no proven connection between any of these factors and the disease develo ... | 2009 | 19748534 |
| studies of porcine circovirus type 2, porcine boca-like virus and torque teno virus indicate the presence of multiple viral infections in postweaning multisystemic wasting syndrome pigs. | in a previous study, using random amplification and large-scale sequencing technology, we identified a novel porcine parvovirus belonging to the genus bocavirus in the background of porcine circovirus type 2 (pcv-2) in swedish pigs with postweaning multisystemic wasting syndrome (pmws). in addition to bocavirus we demonstrated the presence of torque teno virus (ttv) genogroups 1 and 2 in these cases of pmws, indicating the simultaneous presence of several viruses in this disease complex. in the ... | 2010 | 20542066 |
| development of a novel taqman-based real-time pcr assay for the detection of porcine boca-like virus (pbo-likev). | abstract: the recently discovered porcine boca-like virus (pbo-likev) is a member of the parvoviridae family, genus bocavirus, and it is potentially associated with swine disease. several studies have associated pbo-likev with postweaning multisystemic wasting syndrome in pigs, but the full spectrum of clinical disease and the epidemiology of pbo-likev infection remain unclear. the availability of rapid and reliable molecular diagnostics would aid future studies of this novel virus. thus, we dev ... | 2011 | 21771316 |
| porcine bocaviruses: genetic analysis and prevalence in chinese swine population. | summaryin members of the bocavirus genus, that contain three open reading frames (orfs) of the parvovirinae subfamily, porcine bocaviruses (pobovs) exhibit the most genetic diversity. based on the orf2-encoded viral protein (vp1) classification, the six reported porcine bocaviruses were grouped into four species: pobov1 (porcine boca-like virus or pbolv), pobov2 (porcine parvovirus 4 or ppv4), pobov3 (pbov1/pbov2) and pobov4 (6v/7v), with pobov3 and pobov4 each having two genotype viruses. all f ... | 2011 | 21676363 |
| development of a loop-mediated isothermal amplification method for rapid detection of porcine boca-like virus. | the porcine boca-like virus (pbo-likev) was recently discovered in swedish pigs with post-weaning multisystemic wasting syndrome (pmws). in this study, a loop-mediated isothermal amplification (lamp) assay was developed for rapid, specific and sensitive detection of pbo-likev. a set of four primers specific for six regions of pbo-likev vp1/2 genes was designed with the online software. the reaction temperature and time were optimized to 65°c and 60min, respectively. lamp products were detected b ... | 2011 | 22172971 |
| detection of a porcine boca-like virus in combination with porcine circovirus type 2 genotypes and torque teno sus virus in pigs from postweaning multisystemic wasting syndrome (pmws)-affected and non-pmws-affected farms in archival samples from great britain. | in this study we detail the detection and genetic analysis of a novel porcine boca-like virus (pbo-likev) in archival sera and tissue samples from pigs from farms in great britain. we also investigate the distribution of porcine circovirus type 2 (pcv2) genotypes and torque teno sus virus (ttsuv) genogroups 1 and 2 in combination with this novel pbo-likev. pbo-likev was detected in over 70% of all tissues investigated. over 24% of all tissues recovered from pmws-affected animals had all viruses ... | 2013 | 23578709 |