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complete genome sequence of porcine parvovirus 2 recovered from swine sera.a complete genomic sequence of porcine parvovirus 2 (ppv-2) was detected by viral metagenome analysis on swine sera. a phylogenetic analysis of this genome reveals that it is highly similar to previously reported north american ppv-2 genomes. the complete ppv-2 sequence is 5,426 nucleotides long.201626823583
detection of porcine parvovirus 2 (ungulate tetraparvovirus 3) specific antibodies and examination of the serological profile of an infected swine herd.porcine parvovirus 2 (ppv2) is a member of a recently discovered group of swine parvoviruses occurring worldwide. it is frequently detected in lung samples suggesting some pathological role of the virus in diseases. to study this possibility an indirect elisa was developed to detect ppv2 specific antibodies and to examine the serological profile of an infected swine herd where 185 serum samples collected from different age groups including sows were analyzed. according to the results maternal an ...201626974825
coexistence of multiple strains of porcine parvovirus 2 in pig farms.the porcine parvovirus 2 (ppv2) genome was first identified in 2001 in myanmar. recently, the ppv2 genome has been found in several other countries. in this study, the prevalence of ppv2 in japanese domestic pigs was investigated and found to be 58% (69/120) in healthy domestic pigs and 100% (69/69) in sick domestic pigs. sequencing and phylogenetic analysis of the pcr products of the vp1 gene and an almost full length ppv2 clone indicated that diverged ppv2 strains exist in japan. clearly disti ...201424845822
emerging novel porcine parvoviruses in europe: origin, evolution, phylodynamics and phylogeography.to elucidate the spatiotemporal phylodynamics, dispersion and evolutionary processes underlying the emergence of novel porcine parvovirus 2 (ppv2), ppv3 and ppv4 species, we analysed all available complete capsid genes, together with ours, obtained in europe. bayesian phylogeography indicates that romania (ppv2 and ppv4) and croatia (ppv3) are the most likely ancestral areas from which ppvs have subsequently spread to other european countries and regions. the timescale of our reconstruction supp ...201323884365
characterization of porcine parvovirus type 2 (ppv2) which is highly prevalent in the usa.a novel porcine parvovirus designated as porcine parvovirus 2 (ppv2) was initially identified in myanmar in 2001, in china during 2006-2007, and in hungary in 2012. to investigate the presence and prevalence of ppv2 in the usa, a novel taqman(®) real-time pcr method was developed and used for a ppv2 survey using 483 lung samples, 185 fecal samples and 122 sera collected from pigs on 295 farms in 18 u.s. states. the overall prevalence of ppv2 was 20.7% (100/483) in lung samples and 7.6% (14/185) ...201322901530
a viral metagenomic approach on a non-metagenomic experiment: mining next generation sequencing datasets from pig dna identified several porcine parvoviruses for a retrospective evaluation of viral infections.shot-gun next generation sequencing (ngs) on whole dna extracted from specimens collected from mammals often produces reads that are not mapped (i.e. unmapped reads) on the host reference genome and that are usually discarded as by-products of the experiments. in this study, we mined ion torrent reads obtained by sequencing dna isolated from archived blood samples collected from 100 performance tested italian large white pigs. two reduced representation libraries were prepared from two dna pools ...201728662150
complete genome sequences of porcine parvovirus 2 isolated from swine in the republic of korea.here, we report sequences of the porcine parvovirus 2 (ppv2) genome, isolated from pigs in the republic of korea in 2016. the sequences of open reading frames 1 and 2 (orf1 and orf2) had a 98.8 to 98.9% homology with the us135 strain and 98.8% homology with the us523 strain, respectively. this is the first study to report the ppv2 genome in south korean pigs.201728408693
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