| biological and molecular characterization of tospoviruses in thailand. | twenty-eight isolates of tospoviruses associated with tomato, pepper, cucurbits, peanut, and physalis plants collected from fields in different regions of thailand were characterized. on the basis of n gene and protein sequence relationships, three tospoviruses were identified, namely watermelon silver mottle virus (wsmov), capsicum chlorosis virus (cacv), and melon yellow spot virus (mysv). clustal analysis of selected n protein sequences showed different isolates of cacv in three distinct clad ... | 2008 | 18188501 |
| melon yellow spot virus: a distinct species of the genus tospovirus isolated from melon. | abstract a tospovirus-like virus recovered from netted melon was transmitted by thrips palmi in a persistent manner but had different cytopathological features from tospoviruses previously reported. viral nucleocapsid (n) was purified with two protective reagents, 2-mercaptoethanol and l-ascorbic acid, and rna extracted from the viral nucleocapsid was used for genomic analysis. the virus had a genome consisting of three single-stranded rna molecules. the open reading frame on the viral complemen ... | 2000 | 18944594 |
| tomato necrotic ringspot virus, a new tospovirus isolated in thailand. | a new tospovirus isolated from naturally infected tomato plants grown in nakhon pathom province (thailand) was characterized. infected plants showed symptoms consisting of necrotic spots, necrotic ringspots and stem necrosis. this virus was detected using general antibodies that could recognize watermelon silver mottle virus (wsmov), capsicum chlorosis virus (cacv) and melon yellow spot virus (mysv). however, it did not react with specific monoclonal antibodies (mabs) to wsmov and cacv or a spec ... | 2010 | 21104282 |
| development of a protocol for the identification of tospoviruses and thrips species in individual thrips. | a protocol for identifying tospovirus and thrips species in an individual thrips sample was successfully developed. first, an individual thrips was soaked in an rna stabilization solution to preserve protein and nucleic acids and ground in a carbonate buffer containing 0.2% sodium diethyldithiocarbamate. initially, the thrips extracts were screened for tospovirus infection by dot blot analysis using antibodies to nucleocapsid (n) proteins of tospoviruses. thrips extracts with positive results by ... | 2015 | 26141731 |