| a simple wax-embedding method for isolation of aphid hemolymph for detection of luteoviruses in the hemocoel. | a protocol for isolating hemolymph from viruliferous aphids has been developed. this method uses warm melted wax to immobilize the aphid. following removal of a hind leg, the hemolymph can be collected readily. flushing with rnase-free water allows for collection of sufficient hemolymph for rna extraction from individual aphids. the extracted rna was successfully used for detection of barley yellow dwarf virus (bydv) and pea enation mosaic virus (pemv) from individual viruliferous rhopalosiphum ... | 2006 | 16307802 |