| a novel insect-specific flavivirus replicates only in aedes-derived cells and persists at high prevalence in wild aedes vigilax populations in sydney, australia. | to date, insect-specific flaviviruses (isfs) have only been isolated from mosquitoes and increasing evidence suggests that isfs may affect the transmission of pathogenic flaviviruses. to investigate the diversity and prevalence of isfs in australian mosquitoes, samples from various regions were screened for flaviviruses by elisa and rt-pcr. thirty-eight pools of aedes vigilax from sydney in 2007 yielded isolates of a novel flavivirus, named parramatta river virus (parv). sequencing of the viral ... | 2015 | 26519596 |