| insect-mediated transmission of mixed and reassorted cucumovirus genomic rnas. | transmissions of virus using the aphid myzus persicae were performed using plants co-infected with two cucumoviruses, tomato aspermy virus (v-tav) and cucumber mosaic virus (m-cmv). five of the aphid-transmitted progeny viruses (3.7%) induced symptoms distinct from those induced by either parental virus. northern blot hybridization analysis of encapsidated rnas from these novel progeny demonstrated that all of the rna profiles were characteristic of pseudo-recombinants, i.e. viruses with reassor ... | 1992 | 1645147 |
| amino acid changes in the coat protein of cucumber mosaic virus differentially affect transmission by the aphids myzus persicae and aphis gossypii. | cucumber mosaic virus (cmv) transmission by two aphid species was investigated using strains of the virus with modified coat proteins. chimeric viral rna 3 cdna constructs were designed with coat protein-encoding sequences from the efficiently aphid-transmitted fny-cmv and the very poorly aphid-transmitted m-cmv. different regions of the viral coat protein variably influenced transmission; the magnitude of effects depended on the aphid species used as vector. five amino acid changes in the coat ... | 1998 | 9501042 |
| cucumber mosaic virus mutants with altered physical properties and defective in aphid vector transmission. | two mutant strains of cucumber mosaic virus (cmv) were investigated with respect to virion stability and molecular determinants of aphid vector transmission. the mutant 2a1-mt-60x, derived from the mechanically passaged wild type 2a1-at, is poorly transmissible by the aphid aphis gossypii and not transmissible by the aphid myzus persicae, whereas the wild type virus is transmissible by both aphid species. the mutant phenotype was shown to be conferred by a single encoded amino acid change of ala ... | 2000 | 11040130 |
| analysis of genetic bottlenecks during horizontal transmission of cucumber mosaic virus. | genetic bottlenecks may occur in virus populations when only a few individuals are transferred horizontally from one host to another, or when a viral population moves systemically from the infection site. genetic bottlenecks during the systemic movement of an rna plant virus population were reported previously (h. li and m. j. roossinck, j. virol. 78:10582-10587, 2004). in this study we mechanically inoculated an artificial population consisting of 12 restriction enzyme marker mutants of cucumbe ... | 2006 | 16912285 |
| transmission efficiency of cucumber mosaic virus by aphids associated with virus epidemics in snap bean. | cucumber mosaic virus (cmv) is a major component of the virus complex that has become more pronounced in snap bean in the midwestern and northeastern united states since 2001. multiple-vector-transfer tests were done to estimate the cmv transmission efficiencies (p) of the main aphid species identified in commercial snap bean fields in new york and pennsylvania. the four most efficient vectors (p > 0.05) were aphis gossypii, a. glycines, acyrthosiphon pisum, and therioaphis trifolii, which were ... | 2008 | 18943413 |
| capsid protein of cowpea chlorotic mottle virus is a determinant for vector transmission by a beetle. | cowpea chlorotic mottle virus (ccmv) is a bromovirus transmitted by species of chrysomelid beetles, including the spotted cucumber beetle, diabrotica undecimpunctata howardii barber. an experimental system was set up to identify the viral determinant(s) of the beetle transmission of ccmv. nicotiana clevelandii was selected as an experimental plant host because it supports the replication and accumulation of both ccmv and a second member of the family bromoviridae, cucumber mosaic virus (cmv). us ... | 2010 | 19828763 |
| deceptive chemical signals induced by a plant virus attract insect vectors to inferior hosts. | previous studies have shown that vector-borne pathogens can alter the phenotypes of their hosts and vectors in ways that influence the frequency and nature of interactions between them, with significant implications for the transmission and spread of disease. for insect-borne pathogens, host odors are particularly likely targets for manipulation, because both plant- and animal-feeding insects use volatile compounds derived from their hosts as key foraging cues. here, we document the effects of a ... | 2010 | 20133719 |
| elevated co2 shifts the focus of tobacco plant defences from cucumber mosaic virus to the green peach aphid. | elevation in co(2) concentration broadly impacts plant physiological characteristics, which influences herbivores and biotrophic pathogens, which in turn regulate the plant defensive response. in this study, responses of tobacco plants to stress in the form of the green peach aphid, myzus persicae (sulzer), or cucumber mosaic virus (cmv), or both aphid and cmv combined were investigated in open-top chambers under ambient and elevated co(2) concentrations. we measured aboveground biomass and foli ... | 2010 | 20626646 |
| cucumber mosaic virus and its 2b rna silencing suppressor modify plant-aphid interactions in tobacco. | the cucumber mosaic virus (cmv) 2b protein not only inhibits anti-viral rna silencing but also quenches transcriptional responses of plant genes to jasmonic acid, a key signalling molecule in defence against insects. this suggested that it might affect interactions between infected plants and aphids, insects that transmit cmv. we found that infection of tobacco with a 2b gene deletion mutant (cmvδ2b) induced strong resistance to aphids (myzus persicae) while cmv infection fostered aphid survival ... | 2011 | 22355702 |
| infection of host plants by cucumber mosaic virus increases the susceptibility of myzus persicae aphids to the parasitoid aphidius colemani. | plant viruses can profoundly alter the phenotypes of their host plants, with potentially far-reaching implications for ecology. yet few studies have explored the indirect, host-mediated, effects of plant viruses on non-vector insects. we examined how infection of cucurbita pepo plants by cucumber mosaic virus (cmv) impacted the susceptibility of aphids (myzus persicae) to attack by the parasitoid wasp aphidius colemani. in semi-natural foraging assays, we observed higher rates of aphid parasitis ... | 2015 | 26043237 |
| a trio of viral proteins tunes aphid-plant interactions in arabidopsis thaliana. | virus-induced deterrence to aphid feeding is believed to promote plant virus transmission by encouraging migration of virus-bearing insects away from infected plants. we investigated the effects of infection by an aphid-transmitted virus, cucumber mosaic virus (cmv), on the interaction of arabidopsis thaliana, one of the natural hosts for cmv, with myzus persicae (common names: 'peach-potato aphid', 'green peach aphid'). | 2013 | 24349433 |
| the cuticle protein gene mpcp4 of myzus persicae (homoptera: aphididae) plays a critical role in cucumber mosaic virus acquisition. | myzus persicae (sulzer) (homoptera: aphididae) is one of the most important agricultural pests worldwide. in addition to sucking phloem sap, m. persicae also transmits cucumber mosaic virus (cmv) as a vector in a nonpersistent manner. at present, the infection mechanism remains unclear, especially the process of aphid virus acquisition. in this study, we isolated four m. persicae cuticle protein genes (mpcp1, mpcp2, mpcp4, and mpcp5) from m. persicae. the relative amount of the gene encoding cuc ... | 2017 | 28334092 |
| a genetically novel, narrow-host-range isolate of cucumber mosaic virus (cmv) from rosemary. | an isolate of cucumber mosaic virus (cmv), designated cmv-rom, was isolated from rosemary (rosmarinus officinalis) plants in several locations near avignon, france. laboratory studies showed that, unlike typical cmv isolates, cmv-rom has a particularly narrow host range. it could be transmitted by aphids aphis gossypii and myzus persicae, but with low efficacy compared to a typical cmv isolate. phylogenetic analysis of the nucleotide sequences of the cmv-rom genomic rnas shows that this isolate ... | 2016 | 27138549 |
| aphid performance changes with plant defense mediated by cucumber mosaic virus titer. | cucumber mosaic virus (cmv) causes appreciable losses in vegetables, ornamentals and agricultural crops. the green peach aphid, myzus persicae sulzer (aphididae) is one of the most efficient vectors for cmv. the transmission ecology of aphid-vectored cmv has been well investigated. however, the detailed description of the dynamic change in the plant-cmv-aphid interaction associated with plant defense and virus epidemics is not well known. | 2016 | 27103351 |
| elevated co2 impacts bell pepper growth with consequences to myzus persicae life history, feeding behaviour and virus transmission ability. | increasing atmospheric carbon dioxide (co2) impacts plant growth and metabolism. indirectly, the performance and feeding of insects is affected by plant nutritional quality and resistance traits. life history and feeding behaviour of myzus persicae were studied on pepper plants under ambient (aco2, 400 ppm) or elevated co2 (eco2, 650 ppm), as well as the direct impact on plant growth and leaf chemistry. plant parameters were significantly altered by eco2 with a negative impact on aphid's life hi ... | 2016 | 26743585 |
| visual monitoring of cucumber mosaic virus infection in nicotiana benthamiana following transmission by the aphid vector myzus persicae. | the single-stranded, positive-sense and tripartite rna virus cucumber mosaic virus (cmv) was used in this study as a method for monitoring the initial stages of virus infection following aphid transmission. the rna2 of cmv was modified to incorporate, in a variety of arrangements, an open reading frame (orf) encoding an enhanced green fluorescent protein (egfp). the phenotypes of five engineered rna2s were tested in nicotiana tabacum, nicotiana clevelandii and nicotiana benthamiana. only one con ... | 2015 | 25979730 |
| control of insect vectors and plant viruses in protected crops by novel pyrethroid-treated nets. | long-lasting insecticide-treated nets (llitns) constitute a novel alternative that combines physical and chemical tactics to prevent insect access and the spread of insect-transmitted plant viruses in protected enclosures. this approach is based on a slow-release insecticide-treated net with large hole sizes that allow improved ventilation of greenhouses. the efficacy of a wide range of llitns was tested under laboratory conditions against myzus persicae, aphis gossypii and bemisia tabaci. two n ... | 2015 | 25404196 |
| the relationship between host lifespan and pathogen reservoir potential: an analysis in the system arabidopsis thaliana--cucumber mosaic virus. | identification of the determinants of pathogen reservoir potential is central to understand disease emergence. it has been proposed that host lifespan is one such determinant: short-lived hosts will invest less in costly defenses against pathogens, so that they will be more susceptible to infection, more competent as sources of infection and/or will sustain larger vector populations, thus being effective reservoirs for the infection of long-lived hosts. this hypothesis is sustained by analyses o ... | 2014 | 25375140 |
| interference with jasmonic acid-regulated gene expression is a general property of viral suppressors of rna silencing but only partly explains virus-induced changes in plant-aphid interactions. | the cucumber mosaic virus (cmv) 2b viral suppressor of rna silencing (vsr) inhibits host responses to jasmonic acid (ja), a chemical signal regulating resistance to insects. previous experiments with a cmv subgroup ia strain and its 2b gene deletion mutant suggested that vsrs might neutralize aphid (myzus persicae) resistance by inhibiting ja-regulated gene expression. to further investigate this, we examined ja-regulated gene expression and aphid performance in nicotiana benthamiana infected wi ... | 2014 | 24362960 |
| cucumber mosaic virus and its 2b protein alter emission of host volatile organic compounds but not aphid vector settling in tobacco. | aphids, including the generalist herbivore myzus persicae, transmit cucumber mosaic virus (cmv). cmv (strain fny) infection affects m. persicae feeding behavior and performance on tobacco (nicotiana tabacum), arabidopsis thaliana and cucurbits in varying ways. in arabidopsis and cucurbits, cmv decreases host quality and inhibits prolonged feeding by aphids, which may enhance virus transmission rates. cmv-infected cucurbits also emit deceptive, aphid-attracting volatiles, which may favor virus ac ... | 2017 | 28468686 |