| maximizing the detection capability of a beet western yellows virus elisa system. | conditions for maximizing detection of a california isolate of beet western yellows virus (bwyv) were investigated with the double-sandwich enzyme-linked immunosorbent assay (elisa) system. within-plate variability was found to account for less than 1% of the total variation observed on individual microtiter plates. variability across plates was greater than within plates and accounted for less than 10% of the total variation. significant reductions in absorbance were observed when either coatin ... | 1984 | 6392314 |
| aphid transmission of beet western yellows luteovirus requires the minor capsid read-through protein p74. | beet western yellows luteovirus is obligately transmitted by the aphid myzus persicae in a circulative, non-propagative fashion. virus movement across the epithelial cells of the digestive tube into the hemocoel and from the hemocoel into the accessory salivary glands is believed to occur by receptor-mediated endocytosis and exocytosis. virions contain two types of protein; the major 22 kda capsid protein and the minor read-through protein, p74, which is composed of the major capsid protein fuse ... | 1995 | 7882968 |
| effects of point mutations in the readthrough domain of the beet western yellows virus minor capsid protein on virus accumulation in planta and on transmission by aphids. | point mutations were introduced into or near five conserved sequence motifs of the readthrough domain of the beet western yellows virus minor capsid protein p74. the mutant virus was tested for its ability to accumulate efficiently in agroinfected plants and to be transmitted by its aphid vector, myzus persicae. the stability of the mutants in the agroinfected and aphid-infected plants was followed by sequence analysis of the progeny virus. only the mutation y201d was found to strongly inhibit v ... | 2000 | 10627524 |
| studies on the role of the minor capsid protein in transport of beet western yellows virus through myzus persicae. | beet western yellows virus (bwyv), family luteoviridae, is an icosahedral plant virus which is strictly transmitted by aphids in a persistent and circulative manner. virions cross two cellular barriers in the aphid by receptor-based mechanisms involving endocytosis and exocytosis. particles are first transported across intestinal cells into the haemolymph and then across accessory salivary gland cells for delivery to the plant via saliva. we identified the midgut part of the digestive tract as t ... | 2001 | 11458007 |
| posterior midgut and hindgut are both sites of acquisition of cucurbit aphid-borne yellows virus in myzus persicae and aphis gossypii. | members of the family luteoviridae ('luteovirids') rely strictly on aphid vectors for plant-to-plant transmission. this interaction operates according to a persistent and circulative manner, which implies that the virions are being endocytosed and exocytosed across two epithelial barriers (alimentary tract and accessory salivary glands) in the vector's body. in several luteovirid-aphid vector species combinations, the route of virions in the insect has been investigated ultrastructurally by tran ... | 2003 | 14645929 |
| rack-1, gapdh3, and actin: proteins of myzus persicae potentially involved in the transcytosis of beet western yellows virus particles in the aphid. | beet western yellows virus (bwyv) is a polerovirus that relies on the aphid myzus persicae for its transmission, in a persistent-circulative mode. to be transmitted, the virus must cross the midgut and the accessory salivary glands (asg) epithelial barriers in a transcytosis mechanism where vector receptors interact with virions. in this paper, we report in vitro interaction experiments between bwyv and aphid components. using the m. persicae clone from colmar, we showed that a set of aphid poly ... | 2004 | 15246278 |
| beet poleroviruses: close friends or distant relatives? | summary taxonomy: there are three members of the genus polerovirus (family luteoviridae) that induce yellowing of sugar beet: beet mild yellowing virus (bmyv), beet chlorosis virus (bchv) and beet western yellows virus-usa (bwyv-usa, fig. 1). non-beet-infecting isolates of bwyv found particularly within europe have now been re-named turnip yellows virus (tuyv). species-specific antibodies are unavailable, but the viruses can be distinguished by rt-pcr using primers specifically designed to the 5 ... | 2005 | 20565633 |