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utility of a set of conserved mitochondrial cytochrome oxidase subunit i gene primers for mansonia annulata identification.dna-based identification system using the mitochondrial cytochrome oxidase subunit i (coi) gene has enabled validation of many species in certain taxonomic groups. these primer combinations were able to work universally across insecta. here, a set of three primer pairs were successful in amplifying coi of mansonia annulata mosquito, a potential vector of brugia malayi. by merging all three amplicons the whole coi was obtained. primer pair ty-j-1460/c1n2087 amplified 5' region of coi, lepf1/lepr1 ...201122299406
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