an ospa antigen-capture enzyme-linked immunosorbent assay for detecting north american isolates of borrelia burgdorferi in larval and nymphal ixodes dammini. | an antigen-capture enzyme-linked immunosorbent assay (elisa) was developed for detecting north american isolates of borrelia burgdorferi in larval, nymphal, and adult ticks. the assay uses an anti-ospa monoclonal antibody (h5332) for antigen capture and biotin-labelled polyclonal sera with streptavidinhorseradish peroxidase for signal generation. the assay recognized 15 of 15 north american b. burgdorferi isolates and did not cross-react with spirochete antigens of borrelia hermsii, borrelia tur ... | 1993 | 8432812 |
improving the specificity of 16s rdna-based polymerase chain reaction for detecting borrelia burgdorferi sensu lato-causative agents of human lyme disease. | 16s rdna sequences of borrelia burgdorferi sensu lato were aligned with the 16s rdna sequences of borrelia hermsii, borrelia turicatae, and borrelia lonestari in order to identify primers that might be used to more specifically identify agents of human lyme disease in ticks in human skin samples. | 2005 | 15752343 |