| rickettsia buchneri sp. nov., a rickettsial endosymbiont of the blacklegged tick ixodes scapularis. | we obtained a rickettsial isolate from the ovaries of the blacklegged tick, ixodes scapularis. the isolate (iso7(t)) was grown in the ixodes ricinus embryonic cell line ire11. we characterized the isolate by transmission electron microscopy and gene sequencing. phylogenetic analysis of 11 housekeeping genes demonstrated that the isolate fulfils the criteria to be classified as a representative of a novel rickettsial species closely related to 'rickettsia monacensis'. these rickettsiae form a cla ... | 2015 | 25563918 |