an ospa antigen-capture enzyme-linked immunosorbent assay for detecting north american isolates of borrelia burgdorferi in larval and nymphal ixodes dammini. | an antigen-capture enzyme-linked immunosorbent assay (elisa) was developed for detecting north american isolates of borrelia burgdorferi in larval, nymphal, and adult ticks. the assay uses an anti-ospa monoclonal antibody (h5332) for antigen capture and biotin-labelled polyclonal sera with streptavidinhorseradish peroxidase for signal generation. the assay recognized 15 of 15 north american b. burgdorferi isolates and did not cross-react with spirochete antigens of borrelia hermsii, borrelia tur ... | 1993 | 8432812 |